Campylobacter spp. is the leading cause of bacterial gastroenteritis worldwide and poultry are the primary reservoir. The aim of this study was to investigate the survival and/or growth of Campylobacter jejuni NCTC 11168 in broiler digestate prepared from commercial starter, grower and finisher feed formulations. Bolton broth and digestates were prepared, inoculated with C. jejuni NCTC 11168 (approximately 3 log10 CFU per ml) and incubated under microaerobic conditions at 42°C for 24 h. Samples were taken at t = 0 (immediately after inoculation) and every 3 h thereafter, serially diluted and plated onto mCCDA. Campylobacter jejuni grew as expected in Bolton broth (control) reaching the early stationary phase after approximately 15 h. In contrast, although bacterial concentrations were maintained for at least 9 h, none of the feed digestates supported the growth of C. jejuni, which were not detected after 15 h. It is suggested that the nutrients available in the feed digestates are not enough to support C. jejuni growth and that additional factors may be at play in the avian gastrointestinal tract.

中文翻译：

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不同饲料类型肉鸡消化物中空肠弯曲杆菌存活和/或生长的体外研究

弯曲杆菌属 是全球细菌性胃肠炎的主要原因，家禽是主要宿主。本研究的目的是调查空肠弯曲杆菌 NCTC 11168 在由商业起始、生长和肥育饲料配方制备的肉鸡消化物中的存活和/或生长。制备 Bolton 肉汤和消化物，用空肠弯曲杆菌 NCTC 11168（每毫升约 3 log10 CFU）接种，并在微需氧条件下在 42°C 下培养 24 小时。在 t = 0（接种后立即）和此后每 3 小时取样，连续稀释并接种到 mCCDA 上。空肠弯曲杆菌在 Bolton 肉汤（对照）中按预期生长，大约 15 小时后达到早期稳定期。相比之下，尽管细菌浓度至少保持了 9 小时，没有一种饲料消化物支持空肠弯曲杆菌的生长，15 小时后未检测到空肠弯曲杆菌。有人认为饲料消化物中可用的营养物质不足以支持空肠弯曲杆菌的生长，其他因素可能在禽类胃肠道中起作用。