Successful human norovirus (HuNoV) cultivation in stem cell‐derived human intestinal enteroids (HIE) was recently reported. The purpose of this study was to evaluate the anti‐HuNoV efficacy of two alcohol‐based commercial hand sanitizers and 60% ethanol by suspension assay using RNase‐RT‐qPCR, with subsequent validation of efficacy by HuNoV cultivation using the HIE model. In suspension, when evaluated by RNase‐RT‐qPCR, 60% ethanol resulted in less than one log10 reduction in HuNoV genome equivalent copies (GEC) regardless of contact time (30 or 60s) or soil load. The two commercial products outperformed 60% ethanol regardless of contact time or soil load, providing 2·2–3·2 log10 HuNoV GEC reductions by suspension assay. Product B could not be validated in the HIE model due to cytotoxicity. Following a 60s exposure, viral replication in the HIE model increased 1·9 ± 0·2 log10 HuNoV GEC for the neutralization (positive) control and increased 0·9 ± 0·2 log10 HuNoV GEC in challenged HIE after treatment with 60% ethanol. No HuNoV replication in HIE was observed after a 60 s exposure to Product A.

中文翻译：

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使用 RNase-RT-qPCR 的酒精类洗手液对抗人类诺如病毒的功效，并通过人类肠道肠内复制进行验证

最近报道了在干细胞衍生的人肠肠样体 (HIE) 中成功培养人类诺如病毒 (HuNoV)。本研究的目的是通过使用 RNase-RT-qPCR 的悬浮测定来评估两种基于酒精的商业洗手液和 60% 乙醇的抗 HuNoV 功效，随后使用 HIE 模型通过 HuNoV 培养验证功效。在悬浮液中，当通过 RNase-RT-qPCR 评估时，无论接触时间（30 或 60 秒）或土壤负荷如何，60% 乙醇都会导致 HuNoV 基因组等效拷贝 (GEC) 减少不到 1 个 log10。无论接触时间或土壤负荷如何，这两种商业产品都优于 60% 乙醇，通过悬浮测定提供 2·2–3·2 log10 HuNoV GEC 减少。由于细胞毒性，产品 B 无法在 HIE 模型中验证。经过 60 年代的曝光，HIE 模型中的病毒复制增加了中和（阳性）对照的 1·9 ± 0·2 log10 HuNoV GEC，并在用 60% 乙醇处理后在受攻击的 HIE 中增加了 0·9 ± 0·2 log10 HuNoV GEC。在暴露于产品 A 60 秒后，未观察到 HIE 中的 HuNoV 复制。