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Encapsulation of Clitoria ternatea extract in liposomes by synergistic combination of probe‐type ultrasonication and high‐pressure processing
Journal of Food Safety ( IF 2.4 ) Pub Date : 2020-09-23 , DOI: 10.1111/jfs.12859
Hua Wei Chen, Yu Wei Chang

Clitoria ternatea petals are commonly used in health drinks and natural food colorants due to their antioxidant and antibacterial effects, as well as preventing cardiovascular diseases. In this study, the extract of Clitoria ternatea petals obtained under different temperature conditions was analyzed for their antioxidant components (polyphenols, flavonoids, anthocyanins) and their antioxidant capacity (DPPH free‐radical scavenging rate). The effects of the homogenization mode, ratio of lecithin to cholesterol, extract concentration of Clitoria ternatea petals, and extraction temperature on the diameter and uniform of liposome were also investigated to protect their active biological activities. Furthermore, the objective of this study was to improve the homogeneous size distribution of liposomes by probe‐type ultrasonication and high‐pressure processing (HPP) under the effect of pressure through hydrostatic pressure. The results showed that the extract of Clitoria ternatea petals for the best antioxidant effect was obtained at 50°C. According to the results of the Taguchi method, the optimal liposome particle size was the 1:0.15 ratio of lecithin to cholesterol, extract concentration was 0.7%, using the homogenization mode of the probe‐type ultrasonication. As a result, HPP was first employed as a useful method for the preparation of liposome and the improvement of particle size and polydispersity index via the preprocessing of probe sonication and showed considerable promise for use in encapsulation in the dairy beverage, pharmaceutical, and cosmetic industries.

中文翻译:

探针型超声处理与高压处理的协同结合将三生Clitoria ternatea提取物包裹在脂质体中

Clitoria ternatea花瓣由于具有抗氧化和抗菌作用,还可以预防心血管疾病,因此常用于健康饮料和天然食用色素。在这项研究中,分析了在不同温度条件下获得的Clitoria ternatea花瓣的提取物的抗氧化剂成分(多酚,类黄酮,花色苷)及其抗氧化能力(DPPH自由基清除率)。均质化模式,卵磷脂与胆固醇的比率,Clitoria ternatea提取物浓度的影响还研究了花瓣,脂质体直径和均匀度的提取温度,以保护其活性生物活性。此外,本研究的目的是在静水压力的作用下,通过探针型超声和高压处理(HPP)改善脂质体的均匀大小分布。结果表明,Clitoria ternatea的提取物在50°C时可获得具有最佳抗氧化作用的花瓣。根据Taguchi方法的结果,使用探针型超声处理的均质模式,最佳脂质体粒径为卵磷脂与胆固醇的比例为1:0.15,提取物浓度为0.7%。结果,HPP首先被用作有用的方法,用于制备脂质体,并通过探针超声处理的预处理来改善粒径和多分散指数,并显示出在乳制品,制药和化妆品工业中封装的巨大前景。
更新日期:2020-09-23
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