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The ontogeny of greater amberjack digestive and antioxidant defence systems under different rearing conditions: A histological and enzymatic approach
Aquaculture Nutrition ( IF 3.5 ) Pub Date : 2020-09-23 , DOI: 10.1111/anu.13128
José A. Pérez 1 , Ioannis E. Papadakis 2 , Nikos Papandroulakis 2 , Lorenzo Cruces 1 , Efthimia Cotou 3 , Enric Gisbert 4 , Antonio Lorenzo 1 , Constantinos C. Mylonas 2 , Covadonga Rodríguez 1
Affiliation  

An overall synchronization of morphological and physiological ontogenetic events of the digestive and antioxidant defence systems occurred in greater amberjack (Seriola dumerili) larvae reared under intensive (INT) or semi‐intensive (MES) conditions for 30 days. The first differentiations of the digestive channel took place at 3–4 days post‐hatch (dph) (3.6–3.7 mm). Differentiation of the endocrine and exocrine pancreas begun at 4–5 dph (4.0–4.1 mm), coinciding with a decrease in carbohydrase activity from egg to the onset of exogenous feeding and the maintenance of bile salt‐activated lipase and total alkaline proteases. The first gastric glands and pepsin activity were evident at 17–20 dph (5.5–6.2 mm) in both fish groups. The appearance of pyloric caeca had a 5‐day delay in the INT compared to the MES larvae (28 versus 23 dph). Antioxidant enzyme activities decreased progressively from 7 to 30 dph in both rearing conditions. However, GPx, SOD at 7 and 18 dph, and GST activities were higher in the INT larvae. Although both larval groups did not globally differ in somatic growth and maturation of digestive function, it seems that the antioxidant defence system of MES larvae had to face less oxidative assaults, which may be attributed to lower stressful and more stable culture conditions.

中文翻译:

不同饲养条件下更大的a鱼消化和抗氧化防御系统的个体发育:一种组织学和酶学方法

消化系统和抗氧化系统的形态学和生理学发生事件的整体同步发生在更大的a鱼中(Seriola dumerili)幼虫在强化(INT)或半强化(MES)条件下饲养30天。消化道的最初分化发生在孵化后(dph)(3.6-3.7 mm)后3-4天。内分泌和外分泌胰腺的分化始于4-5 dph(4.0-4.1毫米),这与从鸡蛋到外源性摄食开始的糖酶活性下降以及胆汁盐激活的脂肪酶和总碱性蛋白酶的维持相一致。两组鱼类中的第一个胃腺和胃蛋白酶活性均在17-20 dph(5.5-6.2 mm)时明显。与MES幼虫相比,幽门盲肠的出现在INT中延迟了5天(28对23 dph)。在两种饲养条件下,抗氧化酶活性均从7 dph逐渐降低。但是,INT幼虫的GPx,7 dph和18 dph的SOD以及GST活性较高。
更新日期:2020-11-18
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