Soybean waste (okara) may be used for sustainable processing of bioactive compounds and development of functional foods. Okara was analyzed for isoflavones, antioxidant, cytotoxic and enzyme inhibitory activities. High performance liquid chromatography analysis showed the presence of daidzein (6.20), daidzin (5.85), genistein (3.65), genistin (2.15), glycitin (1.98), and glycitein (1.17 mg/100 g) in okara. Among all the solvent extracts and isolated compounds, the acetonitrile extract, genistein and daidzein showed maximum antioxidant and anti-inflammatory activities. Okara showed high dietary fiber (56) and crude protein (33) compared to crude fat (8.7) and carbohydrate (3.8 g/100 g DM) composition. Okara extracts and isoflavones showed enzyme inhibitory effects; with IC₅₀ values for urease of 41 ± 1 to 65 ± 2 for okara and 10 ± 1 to 21 ± 1 for isoflavones, with the positive control thiourea (7.1 ± 0.1 μg/mL) and for xanthine oxidase 35 ± 1 to 56 ± 2 for okara and 7.3 ± 0.9 to 16 ± 1 for isoflavones, with the positive control allopurinol (5.4 ± 0.1) μg/mL. In cytotoxic studies, the genistein (5.8 and 3.5, μM/mL) and daidzein (7.2 and 4.2 μM/mL) showed higher cytotoxic effects compared to berberine as the control (10.5 and 9.1) IC₅₀ values, respectively. The results suggested that the okara could be used as a source of nutraceuticals and isoflavones with potential antioxidant, anti-inflammatory, and enzyme inhibitory activities. Individual isoflavones from okara might be studied as possible functional ingredients that can act as natural therapeutic agents for conditions involving the xanthine oxidase and urease enzymes.

大豆废料（豆渣）可用于生物活性化合物的可持续加工和功能性食品的开发。分析了豆渣中的异黄酮，抗氧化剂，细胞毒性和酶抑制活性。高效液相色谱分析表明，豆渣中存在大豆苷元（6.20），大豆苷元（5.85），金雀异黄素（3.65），染料木素（2.15），甘露素（1.98）和甘露素（1.17 mg / 100 g）。在所有溶剂提取物和分离的化合物中，乙腈提取物，染料木黄酮和大豆苷元显示出最大的抗氧化和抗炎活性。与粗脂肪（8.7）和碳水化合物（3.8 g / 100 g DM）组成相比，豆渣显示出高的膳食纤维（56）和粗蛋白（33）。豆渣提取物和异黄酮具有抑制酶的作用。带IC ₅₀豆渣的尿素酶值为41±1至65±2，异黄酮的尿素酶值为10±1至21±1，其中阳性对照硫脲（7.1±0.1μg/ mL）和黄嘌呤氧化酶为35±1至56±2（豆渣）异黄酮为7.3±0.9至16±1，阳性对照为别嘌呤醇（5.4±0.1）μg/ mL。在细胞毒性研究中，与黄连素作为对照（10.5和9.1）相比，染料木黄酮（5.8和3.5，μM/ mL）和黄豆苷元（7.2和4.2μM/ mL）显示出更高的细胞毒性作用IC ₅₀值。结果表明，豆渣可以用作具有潜在抗氧化，抗炎和酶抑制活性的保健食品和异黄酮的来源。豆渣中的异黄酮可能被研究为可能的功能成分，可以作为涉及黄嘌呤氧化酶和脲酶的天然药物。