Purpose

The dog is an important animal model for tear dysfunction diseases, however to-date the electrolyte composition of the dog's tears is unknown. The aim of this study was to analyze the electrolyte content of canine tears and compare it to serum and plasma.

Methods

Tear samples were collected from 18 eyes of 9 dogs. Blood for serum was collected in tubes with no anticoagulants; plasma was obtained by using two different anticoagulants: Citrate-Phosphate-Dextrose (CPD) and heparin. The electrolytes were measured in all samples, analyzed, and compared.

Results

Most of the electrolyte values in tears were statistically different (P < 0.05) from electrolyte values in serum and plasma. Potassium and chloride values were significantly higher in tears compared to serum and plasma, while calcium and phosphate values were significantly lower. Sodium values in tears were higher than in serum and heparinized-plasma, but lower than CPD-plasma. Bicarbonate values were lower in tears compared to serum and heparinized plasma, but was not statistically different than CPD-plasma. While magnesium values were lower in tears compared to serum and heparinized-plasma, the difference was not statistically different.

Conclusions

Herein, we report for the first time the electrolyte composition of the canine tears and its comparison to serum and plasma.

中文翻译：

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正常犬眼泪的电解质成分及其与血清和血浆的比较

目的

狗是眼泪功能障碍疾病的重要动物模型，但是迄今为止，狗眼泪的电解质成分尚不清楚。这项研究的目的是分析犬眼泪的电解质含量，并将其与血清和血浆进行比较。

方法

从9只狗的18只眼中收集眼泪样品。用于血清的血液收集在没有抗凝剂的试管中。通过使用两种不同的抗凝剂获得血浆：柠檬酸盐-磷酸盐-葡萄糖（CPD）和肝素。测量所有样品中的电解质，进行分析和比较。

结果

眼泪中的大多数电解质值与血清和血浆中的电解质值有统计学差异（P <0.05）。与血清和血浆相比，眼泪中的钾和氯化物值显着较高，而钙和磷酸盐的值显着较低。眼泪中的钠值高于血清和肝素化血浆，但低于CPD血浆。与血清和肝素化血浆相比，眼泪中的碳酸氢盐值较低，但与CPD血浆在统计学上没有差异。尽管眼泪中的镁含量低于血清和肝素化血浆，但差异无统计学意义。

结论

在此，我们首次报道了犬眼泪的电解质成分及其与血清和血浆的比较。