Abstract

On practical material (samples of wild boar and seven main breeds of domestic pig in Belarus), we confirmed the high differentiating potential of H3GA0051814 polymorphism (HEPH gene). To differentiate between wild boar and domestic pig, we proposed a test model of three SNPs—H3GA0051814 (HEPH), c.367G>A (MC1R), and g.299084751C>T (NR6A1). This model compares favorably with existing approaches, primarily in its versatility (suitable for differentiation, including domestic breeds of pigs of Asian origin), as well as high accuracy (99.14%) and specificity (100%). Using HRM analysis, we developed a quick and simple approach to differentiation based on the proposed test model, which is designed to reduce the time and financial costs of molecular genetic testing, as well as reduce the risk of cross-contamination, because the process is one-stage (the stages of restriction and electrophoresis are excluded).

中文翻译：

---

X染色体上HEPH基因多态性分析以鉴定野猪和家猪

摘要

在实用的材料上（白俄罗斯的野猪和七个主要猪种），我们确认了H3GA0051814多态性（HEPH基因）的高分化潜力。为了区分野猪和家猪，我们提出了三种SNP的测试模型：H3GA0051814（HEPH），c.367G> A（MC1R）和g.299084751C> T（NR6A1））。该模型与现有方法相比具有优势，主要是它的多功能性（适用于分化，包括亚洲血统的国内猪种），并且准确性高（99.14％）和特异性（100％）。使用HRM分析，我们在建议的测试模型的基础上开发了一种快速简单的区分方法，该方法旨在减少分子遗传测试的时间和财务成本，并降低交叉污染的风险，因为该过程非常简单。一阶段（不包括限制和电泳阶段）。