Sustainable management practices are the need of the hour to counter the ever-deteriorating environmental conditions in agro-ecosystems. While bioinoculants have served as eco-friendly means to mitigate stresses, their survivability and competitiveness in field conditions have been a major challenge. The present study aimed to adopt a multi-generational approach to adapt the rhizospheric microbiome of Vigna radiata , a legume consumed worldwide, under salt stress. A multi-generational plant growth experiment was set-up with induced salinity stress, employing the rhizosphere of best-performing plants from the preceding generation as inoculum for the next generation. The plant health and mitigation of salinity stress by the adapted microbiome was analyzed using plant biometrics, bacterial diversity, and stress markers. Decreased levels of salt-induced stress markers, and simultaneous improvement in plant attributes were observed with the progression of the generations. The shifts in bacterial community were prominent upon inoculation of the adapted microbiome. The treatments with the adapted microbiome in the presence of salinity stress were closer to the control sets compared to salinity-stressed treatments, indicating the efficiency of the adapted microbiome towards mitigation of salinity stress. The study demonstrates the promising potential of such an eco-friendly, microbiome-based approach for plant growth promotion and mitigation of salinity stress.

中文翻译：

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通过适应盐胁迫来定制辐射豇豆根际微生物群落

可持续管理实践是应对农业生态系统中不断恶化的环境条件的时代需要。虽然生物接种剂已成为减轻压力的环保手段，但它们在野外条件下的生存能力和竞争力一直是一项重大挑战。本研究旨在采用多代方法来适应盐胁迫下豇豆（一种全世界食用的豆类）的根际微生物群落。建立了具有诱导盐分胁迫的多代植物生长实验，采用上一代表现最佳植物的根际作为下一代接种物。使用植物生物特征、细菌多样性和胁迫标记物分析了适应微生物组对植物健康和盐分胁迫的缓解。随着世代的进展，观察到盐诱导胁迫标记物的水平降低，并且植物属性同时改善。接种适应的微生物组后，细菌群落的变化很明显。与盐胁迫处理相比，在盐胁迫下使用适应微生物组进行的处理更接近于对照组，表明适应微生物组对减轻盐分胁迫的效率。该研究证明了这种基于生态友好、基于微生物组的方法在促进植物生长和减轻盐分胁迫方面的潜力。接种适应的微生物组后，细菌群落的变化很明显。与盐胁迫处理相比，在盐胁迫下使用适应微生物组的处理更接近对照组，表明适应微生物组对减轻盐胁迫的效率。该研究证明了这种基于生态友好、基于微生物组的方法在促进植物生长和减轻盐分胁迫方面的潜力。接种适应的微生物组后，细菌群落的变化很明显。与盐胁迫处理相比，在盐胁迫下使用适应微生物组进行的处理更接近于对照组，表明适应微生物组对减轻盐分胁迫的效率。该研究证明了这种基于生态友好、基于微生物组的方法在促进植物生长和减轻盐分胁迫方面的潜力。