A psychrotolerant Sphingobacterium sp. was isolated from the apple orchard situated in the Kufri region of Shimla, Himachal Pradesh, India using an enrichment culture technique having chlorpyrifos (CP) as the sole source of carbon and energy. Based on biochemical characterization and 16S rRNA analysis, the strain was identified as Sphingobacterium sp. C1B. The bacterium C1B was able to degrade chlorpyrifos ≥ 42 ppm and ≥ 36 ppm within 14 days at 20 °C and 15 °C, respectively. The strain was also able to degrade chlorpyrifos ≤ 35 ppm at 28 °C within 14 days. The enzyme organophosphorus hydrolase might be responsible for the initial degradation of CP by the strain C1B. Based on the HPLC and GCMS analysis, a probable degradation pathway has been proposed, which followed the path from chlorpyrifos to 3,5,6-trichloro-2-pyridinol to benzene, 1,3-bis (1,1-dimethylethyl) and then entered into the TCA cycle. Our current study revealed that the bacterium C1B was found to be a useful strain for the degradation of pesticide chlorpyrifos in the cold climatic environment.

耐精神病菌Sphingobacterium sp。使用富毒栽培技术，以毒死rif（CP）为唯一碳和能量来源，从印度喜马al尔邦西姆拉库弗里州的苹果园中分离得到了苹果。根据生化特征和16S rRNA分析，该菌株被鉴定为Sphingobacteriumsp。C1B。细菌C1B能够在20°C和15°C下在14天内分别降解≥42 ppm和≥36 ppm的毒死rif。该菌株还能够在14天内在28°C下降解≤35 ppm的毒死rif。有机磷水解酶可能是导致菌株C1B降解CP的原因。根据HPLC和GCMS分析，提出了可能的降解途径，该途径遵循从毒死rif到3,5,6-三氯-2-吡啶醇到苯，1,3-双（1,1-二甲基乙基）和然后进入TCA周期。我们目前的研究表明，发现C1B细菌是在寒冷气候环境中降解农药毒死rif的有用菌株。