The present study was designed to propose a simple, cost-effective, and efficient method for the preparation of a biocompatible composite made from magnetic diatomaceous earth (mDE) coated by aminopropyltriethoxysilane (APTES) and its application for immobilization of porcine pancreatic lipase (PPL). The produced mDE-APTES was instrumentally characterized and the obtained results of FTIR analysis and scanning electron microscopy equipped by energy-dispersive X-ray spectroscopy (SEM–EDS) showed successful coating of APTES on mDE surface. PPL was then immobilized onto mDE to obtain the biocatalyst of PPL@mDE (immobilization yield and efficiency of 78.0 ± 0.3% and 80.1 ± 0.6, respectively) and the presence of enzyme was confirmed by EDS method. The attained results of the reusability of PPL@mDE revealed that 57% of the initial activity was retained after 11 cycles of biocatalyst application. PPL@mDE demonstrated higher storage stability than the free enzyme at 4 °C, 25 °C, and 37 °C. The apparent K_m (2.35 ± 0.12 mM) and V_max (13.01 ± 0.64 µmol/min) values for the immobilized enzyme were considerably altered compared to those of the free enzyme (p > 0.05). PPL@mDE was subsequently employed for the synthesis of banana flavor (isoamyl acetate) in n-hexane, which yields an esterification percentage of 100 at 37 °C after 3 h. However, it merits further investigations to find out about large-scale application of the as-synthesized biocatalyst for esterification.

本研究旨在提出一种简单、经济、高效的方法，用于制备由氨丙基三乙氧基硅烷 (APTES) 包覆的磁性硅藻土 (mDE) 制成的生物相容性复合材料及其在固定猪胰脂肪酶 (PPL) 中的应用。 . 对产生的 mDE-APTES 进行仪器表征，获得的 FTIR 分析和配备能量色散 X 射线光谱 (SEM-EDS) 的扫描电子显微镜的结果表明，APTES 成功地涂覆在 mDE 表面上。然后将 PPL 固定在 mDE 上以获得 PPL@mDE 的生物催化剂（固定产率和效率分别为 78.0 ± 0.3% 和 80.1 ± 0.6），并通过 EDS 方法确认酶的存在。PPL@mDE 的可重用性结果表明，在 11 次生物催化剂应用循环后，57% 的初始活性得以保留。PPL@mDE 在 4°C、25°C 和 37°C 下表现出比游离酶更高的储存稳定性。明显的与游离酶相比，固定化酶的K _m (2.35 ± 0.12 mM) 和V _max (13.01 ± 0.64 µmol/min) 值发生了相当大的变化 ( p  > 0.05)。PPL @ MDE随后采用的香蕉味（乙酸异戊酯）的合成Ñ正己烷，其中3小时后产生的100在37℃下进行酯化百分比。然而，值得进一步研究以了解合成的生物催化剂在酯化中的大规模应用。