We aimed to develop an artificial membrane system to observe the infection process of the obligate biotrophic powdery mildew fungi without the use of living plant cells. The conidia of Blumeria graminis and Erysiphe pisi conidia were inoculated on a formvar membrane laid on an artificial medium. Germinated conidia frequently formed appressoria and then penetrated the membrane to form haustorium-like structures in the artificial medium. Secondary hyphae elongation was also observed after the formation of haustorium-like structures. These results suggested that the formvar membrane laid on artificial medium induced the formation of haustorium-like structures that have roles in the formation of secondary hyphae.

我们旨在开发一种人造膜系统，以观察专性的生物营养性白粉病真菌在不使用活植物细胞的情况下的感染过程。将Blumeria graminis分生孢子和Erysiphe pisi分生孢子接种在铺在人造培养基上的formvar膜上。发芽的分生孢子经常形成附生器官，然后穿透膜在人造培养基中形成类似吸痰器的结构。在形成类似吸痰器的结构之后，还观察到次级菌丝伸长。这些结果表明，铺在人造培养基上的formvar膜诱导了在第二菌丝的形成中起作用的类抽烟状结构的形成。