Persistent infection with high-risk human papillomavirus (HR-HPV) is considered as the leading cause of pre-cervical cancer and cancer. Currently, HPV testing is expensive, time-consuming, and requires experienced technicians. Here, we report a method for label-free detection of HR-HPV by recording and analyzing Raman spectroscopy (RS) of cervical exfoliated cell specimens. A total of 315 Raman spectra (165 normal, 150 HR-HPV) were recorded from 63 subjects (33 normal samples, 30 HR-HPV positive patients). Mean Raman spectra differed significantly between normal and HR-HPV positive groups. The differences showed an increase in the relative contents of hemoglobin and tryptophan, and new peaks appeared due to cell-level infection in HR-HPV positive group. Furthermore, a principal component analysis-linear discriminant analysis diagnostic model was developed and applied on the Raman spectra of HR-HPV positive patients as well as normal samples, and satisfactory classification resul...

中文翻译：

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使用拉曼光谱和多变量分析对高危人乳头瘤病毒感染进行无标记检测

持续感染高危型人乳头瘤病毒（HR-HPV）是宫颈癌和癌症的主要原因。当前，HPV测试昂贵，费时，并且需要经验丰富的技术人员。在这里，我们报告通过记录和分析宫颈脱落细胞标本的拉曼光谱（RS），用于无标签检测HR-HPV的方法。从63位受试者（33位正常样本，30位HR-HPV阳性患者）中记录了总共315张拉曼光谱（165张正常，150 HR-HPV）。正常和HR-HPV阳性组之间的平均拉曼光谱差异显着。差异显示HR-HPV阳性组中血红蛋白和色氨酸的相对含量增加，并且由于细胞水平感染而出现新的峰。此外，