Background Hepatocellular carcinoma (HCC) is one of the cancers of highest incidence and mortality worldwide. The proliferation and invasion of tumor cells are the main reason for poor prognosis after HCC surgery. Long non-coding RNA (lncRNA) has been shown to play a key role in the progression of HCC. LncRNA-CR594175 is one of the highly expressed lncRNAs in HCC tumors and their metastatic tumors that we have obtained by the High-throughput screening method. Methods To elucidate the role of lncRNA-CR594175 in regulating the proliferation and invasion of human hepatoma cell line, HepG2, we operated through lncRNA-CR594175 silencing to inhibit the progression of HCC, either through in vitro or in vivo experiments. Results We found that lncRNA-CR594175 was lower in adjacent non-cancerous tissues than in primary HCC, and was lower in primary HCC than in its metastasis. Silencing of lncRNA-CR594175 inhibited the proliferation and invasion of HepG2 cells and growth of subcutaneous tumors. The results revealed that lncRNA-CR594175, as a RNA sponge, broke the negative regulation of hsa-miR-142-3p on Catenin, beta-1 (CTNNB1), and once lncRNA-CR594175 was silenced, the hsa-miR142-3p regained its negative regulation on CTNNB1 which can promote HCC progression by activating the wnt pathway. Conclusions Our present study demonstrated for the first time that lncRNA-CR594175 silencing suppressed proliferation and invasion of HCC cells in vivo and in vitro by restoring the negative regulation of hsa-miR-142-3p on CTNNB1, laying a solid theoretical base for using lncRNA-CR594175 as genetic target therapy for HCC and offering a reasonable explanation for inactivation of miRNA in different tumors or in the tumor at different stages.

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lncRNA-CR594175在体内外调控肝癌细胞增殖和侵袭的机制研究

背景 肝细胞癌 (HCC) 是全球发病率和死亡率最高的癌症之一。肿瘤细胞的增殖和侵袭是HCC术后预后不良的主要原因。长链非编码 RNA (lncRNA) 已被证明在 HCC 的进展中起关键作用。LncRNA-CR594175是我们通过高通量筛选方法获得的HCC肿瘤及其转移性肿瘤中高表达的lncRNA之一。方法 为了阐明 lncRNA-CR594175 在调节人肝癌细胞 HepG2 增殖和侵袭中的作用，我们通过体外或体内实验通过 lncRNA-CR594175 沉默来抑制 HCC 的进展。结果 我们发现邻近非癌组织中的 lncRNA-CR594175 低于原发性 HCC，并且在原发性HCC中低于其转移。lncRNA-CR594175的沉默抑制了HepG2细胞的增殖和侵袭以及皮下肿瘤的生长。结果表明，lncRNA-CR594175作为RNA海绵打破了hsa-miR-142-3p对Catenin, beta-1 (CTNNB1)的负调控，一旦lncRNA-CR594175被沉默，hsa-miR142-3p又恢复了。其对 CTNNB1 的负调控可通过激活 wnt 通路促进 HCC 进展。结