当前位置: X-MOL 学术Eur. J. Mass Spectrom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Shedding light on isomeric FAHFA lipid structures using 213 nm ultraviolet photodissociation mass spectrometry
European Journal of Mass Spectrometry ( IF 1.3 ) Pub Date : 2020-09-22 , DOI: 10.1177/1469066720960341
Edgar White Buenger 1 , Gavin E Reid 1, 2, 3
Affiliation  

Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) are a recently discovered class of biological active lipids with anti-diabetic and anti-inflammatory functions. Given that structure and function are intimately related, we report here the use of direct infusion multi-stage hybrid tandem mass spectrometry involving sequential Collisional Activated Dissociation (CAD) and 213 nm UltraViolet PhotoDissociation (UVPD), as a novel technique for the unambiguous denovo identification and detailed structural characterisation of FAHFA lipid ions, including determination of the esterified fatty acid identity, the hydroxy fatty acid identity and position of esterification, and localization of the site(s) of endogenous unsaturations, without need for chromatographic separation or authentic reference standards. The utility of this approach is demonstrated for the identification of individual FAHFA lipids introduced to the mass spectrometer in positive ionization mode as their lithiated adducts, as well as from mixtures containing isomeric FAHFA species with differing esterification sites, including those that are not resolved by current liquid chromatography methods.

中文翻译:

使用 213 nm 紫外光解质谱法揭示异构 FAHFA 脂质结构

羟基脂肪酸的脂肪酸酯 (FAHFA) 是最近发现的一类具有抗糖尿病和抗炎功能的生物活性脂质。鉴于结构和功能密切相关,我们在此报告使用直接注入多级混合串联质谱法,涉及顺序碰撞激活解离 (CAD) 和 213 nm 紫外光解离 (UVPD),作为一种用于明确从头识别的新技术FAHFA 脂质离子的详细结构表征,包括确定酯化脂肪酸身份、羟基脂肪酸身份和酯化位置,以及内源性不饱和位点的定位,无需色谱分离或可靠的参考标准。
更新日期:2020-09-22
down
wechat
bug