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Improving Fungal Decay Resistance of Less Durable Sapwood by Impregnation with Scots Pine Knotwood and Black Locust Heartwood Hydrophilic Extractives with Antifungal or Antioxidant Properties
Forests ( IF 2.9 ) Pub Date : 2020-09-22 , DOI: 10.3390/f11091024
Viljem Vek , Angela Balzano , Ida Poljanšek , Miha Humar , Primož Oven

Research Highlights: The antifungal assay confirmed that knotwood extractives of Scots pine inhibit the growth of wood decay fungi. Heartwood extracts of black locust were found to be much stronger free radical scavengers than the extracts of Scots pine. The extracts were deposited in the lumina and on the wall surface of cells in the impregnated sapwood. Impregnation of the sapwood blocks with Scots pine and black locust extracts reduced the fungal decay of wood. Objectives: Hydrophilic extracts of Scots pine knotwood and black locust heartwood were chemically analyzed, tested for antifungal and antioxidant properties and used for impregnation of beech and Scots pine sapwood. Materials and Methods: Scots pine knotwood and black locust heartwood were extracted, and obtained hydrophilic extractives were chemically analyzed. Extracts were analyzed for antifungal properties with the in vitro well-diffusion method. The free radical scavenging activity of wood extracts was measured colorimetrically. The retention of the extracts in the impregnated sapwood blocks was evaluated with microscopy and gravimetry. A decay test was performed with the mini block test. Results: Almost half of both Scots pine knotwood and black locust heartwood hydrophilic extracts obtained were described by phenolic compounds. The extracts were deposited in the lumina of cells and on the cell wall surface. Extractives of Scots pine knotwood had good inhibitory properties against white- and brown-rot fungi. On the other hand, extractives of black locust heartwood were found to be good radical scavengers, better than knotwood extractives of Scots pine. The extracts of Scots pine knotwood and black locust reduced the fungal decay of the tested sapwood blocks. Conclusions: The results of this research show that the less-valued knotwood of Scots pine and heartwood of black locust are a potential source of antifungal and antioxidant agents for bio-based wood preservatives.

中文翻译:

通过浸渍具有抗真菌或抗氧化特性的苏格兰松树胶合板和刺槐心材亲水性提取物来提高耐久的边材的抗真菌腐烂性

研究重点:抗真菌试验证实,苏格兰松树的结木提取物可以抑制木材腐烂真菌的生长。发现刺槐的心材提取物比苏格兰松树的提取物具有更强的自由基清除剂。提取物沉积在腔室和浸渍边材的细胞壁表面上。苏格兰松和黑刺槐提取物对边材块的浸渍减少了木材的真菌腐烂。目的:化学分析苏格兰松节材和刺槐心材的亲水性提取物,测试其抗真菌和抗氧化性能,并用于浸渍山毛榉和苏格兰松材。材料与方法:提取樟子松花结木和刺槐心材,对所得亲水性提取物进行化学分析。用体外良好扩散方法分析提取物的抗真菌特性。比色法测量木材提取物的自由基清除活性。用显微镜和重量分析法评估提取物在浸渍边材块中的保留率。用微型块测试进行衰减测试。结果:所获得的苏格兰松节材和刺槐心材亲水性提取物中几乎有一半是酚类化合物。提取物沉积在细胞腔中和细胞壁表面上。苏格兰松节材的提取物对白腐和褐腐真菌具有良好的抑制作用。另一方面,发现刺槐心材提取物是良好的自由基清除剂,优于苏格兰松树的结节材提取物。苏格兰松节材和刺槐的提取物减少了测试的边材块的真菌腐烂。结论:这项研究的结果表明,价值较低的苏格兰松树结皮和刺槐心材是生物基木材防腐剂的抗真菌和抗氧化剂的潜在来源。
更新日期:2020-09-22
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