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Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge
Journal of Environmental Science and Health, Part A ( IF 2.1 ) Pub Date : 2020-09-22
Hyung-Joo Park, Ji Hyeon Kwon, Jeonghee Yun, Kyung-Suk Cho

A new nitrous oxide (N2O)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and N2O as the sole nitrogen source. The isolated facultative anaerobe was identified as Azospira sp. HJ23. Azospira sp. HJ23 exhibited optimum N2O-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for N2O reduction was a mixture of glucose and acetate. The maximum rate of N2O reduction by Azospira sp. HJ23 was 4.8 mmol·g-dry cell−1·h−1, and its N2O-reducing activity was higher than other known N2O reducers. Azospira sp. HJ23 possessed several functional genes for denitrification. These included narG (NO3 - reductase), nirK (NO2 - reductase), norB (NO reductase), and nosZ (N2O reductase) genes. These results suggest that Azospira sp. HJ23 can be applied in the denitrification process to minimalize N2O emission.



中文翻译:

Azospira sp。一氧化二氮还原的表征。从高级废水处理污泥中分离出HJ23

从一个财团中分离出一种新型的减少一氧化二氮(N 2 O)的细菌,该财团使用高级废水处理污泥作为接种物,并以N 2 O作为唯一的氮源进行了浓缩。分离的兼性厌氧菌被鉴定为Azospira sp。HJ23。Azospira SP。HJ23在37°C至40°C的温度范围内,在pH值为6时,具有最佳的N 2 O还原活性,C / N比为62。还原N 2 O的最佳碳源是葡萄糖和乙酸盐的混合物。Azospira sp。的最大N 2 O还原速率。HJ23为4.8 mmol·g干电池-1 ·h -1及其N 2。还原O的活性高于其他已知的N 2 O还原剂。Azospira SP。HJ23具有几个反硝化功能基因。这些包括narG(NO 3 -还原酶),nirK(NO 2 -还原酶),NORB(NO还原酶),并nosZ(N 2 ö还原酶)的基因。这些结果表明,Azospira sp。HJ23可用于反硝化过程,以最大程度地减少N 2 O排放。

更新日期:2020-09-22
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