Animal Biotechnology ( IF 3.7 ) Pub Date : 2020-09-22 , DOI: 10.1080/10495398.2020.1823401 Jinbo Wu 1 , Shiming He 1 , Zhonghua Yu 1 , Daoliang Lan 2 , Xianrong Xiong 2 , Zhu Li 1
Abstract
Yak milk, a high-quality milk, is one of the best raw materials for dairy products and economically important to pastoral herdsmen. To make a further understanding of the molecular differences in mammary tissues of the yaks with different milk production during lactation, in this study, we took the use of RNA-seq to perform high-throughput sequencing and analysis of the mammary gland transcriptomes of both high-yielding yak and low-yielding yaks during lactation. By the comparison and analysis of the transcriptome data for the mammary gland tissue of high-yielding yak and low-yield yak, 144 differential genes were screened out, of which 49 were upregulated and 95 were downregulated. Further functional analysis indicated that these differential genes involved in multiple classes based on Gene Ontology (GO) and multiple Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The GO analysis showed that the functions of the differential genes are closely related to the carbohydrate metabolism and other biological processes. KEGG pathway analysis revealed that these genes are mostly enriched in the pathway of antigen processing and presentation, phagosome pathway and type I diabetes pathway and enriched followed by extracellular matrix receptor interaction pathway. Moreover, several other pathways related to amino acid metabolism also showed significant enrichment. Here, the mammary gland transcriptomes of high-yielding yak and low-yielding yaks during lactation have for the first time been compared, and the related differential genes have been screened out and analyzed. Our study paves a way for the further elucidation of the basic molecular mechanism of yak mammary gland tissue, and at the same time provides new ideas for improving the milk production of yaks.
中文翻译:
牦牛哺乳期乳腺组织的转录组学研究
摘要
牦牛奶是一种优质牛奶,是乳制品的最佳原料之一,对牧民具有重要的经济意义。为了进一步了解泌乳期不同产奶量的牦牛乳腺组织的分子差异,本研究利用RNA-seq对两种高产奶牛的乳腺转录组进行高通量测序和分析。 - 泌乳期高产牦牛和低产牦牛。通过对高产牦牛和低产牦牛乳腺组织转录组数据的比较分析,筛选出144个差异基因,其中上调49个,下调95个。进一步的功能分析表明,这些差异基因涉及基于基因本体论(GO)和多个京都基因和基因组百科全书(KEGG)途径的多个类别。GO分析表明,差异基因的功能与碳水化合物代谢等生物过程密切相关。KEGG通路分析显示,这些基因主要富集在抗原加工和呈递通路、吞噬体通路和I型糖尿病通路中,其次是细胞外基质受体相互作用通路。此外,与氨基酸代谢相关的其他几种途径也显示出显着的富集。在这里,首次比较了泌乳期高产牦牛和低产牦牛的乳腺转录组,并对相关的差异基因进行了筛选和分析。我们的研究为进一步阐明牦牛乳腺组织的基本分子机制铺平了道路,同时为提高牦牛产奶量提供了新思路。