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Endocytosis of BRASSINOSTEROID INSENSITIVE1 is Partly Driven by a Canonical Tyrosine-based Motif.
The Plant Cell ( IF 11.6 ) Pub Date : 2020-11-01 , DOI: 10.1105/tpc.20.00384
Derui Liu 1, 2 , Rahul Kumar 1, 2 , Lucas A N Claus 1, 2 , Alexander J Johnson 3 , Wei Siao 1, 2 , Isabelle Vanhoutte 1, 2 , Peng Wang 1, 2 , Kyle W Bender 4 , Klaas Yperman 1, 2 , Sara Martins 5 , Xiuyang Zhao 1, 2 , Grégory Vert 5 , Daniël Van Damme 1, 2 , Jiří Friml 3 , Eugenia Russinova 2, 6
Affiliation  

Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles via the recognition of motifs based on Tyr or di-Leu in their cytoplasmic tails. However, in plants, very little is known about how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis (Arabidopsis thaliana), the brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) undergoes endocytosis, which depends on clathrin and AP-2. Here, we demonstrate that BRI1 binds directly to the medium AP-2 subunit (AP2M). The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed Tyr-based endocytic motifs. The Tyr-to-Phe substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2–dependent internalization of PM proteins via the recognition of functional Tyr motifs also operates in plants.



中文翻译:

BRASSINOSTEROID INSENSITIVE1的胞吞作用部分由基于典型酪氨酸的母体驱动。

网格蛋白介导的内吞作用(CME)及其核心内吞机制在所有真核生物中都得到了进化保守。在哺乳动物中,异四聚体衔接蛋白复合物2(AP-2)通过识别基于Tyr或di-Leu的胞质尾巴中的基序,将质膜(PM)货物分类为囊泡。然而,在植物中,关于如何对CME分选PM蛋白以及是否需要类似的基序知之甚少。在拟南芥(Arabidopsis thaliana)中,油菜素类固醇(BR)受体BR INSENSITIVE1(BRI1)经历内吞作用,这取决于网格蛋白和AP-2。在这里,我们证明BRI1直接绑定到中等AP-2亚基(AP2M)。BRI1的胞质结构域包含五个假定的典型的基于表面的Tyr基内吞基序。Y中的Tyr-Phe取代898 KAI减少了BRI1的内在化,而没有影响其激酶活性。一致地,携带BRI1 Y898F突变的植物对BR过敏。我们的研究表明,通过识别功能性Tyr基序,依赖AP-2的PM蛋白内在化也可在植物中发挥作用。

更新日期:2020-11-03
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