A new method for simultaneously determining five polycyclic aromatic hydrocarbons (PAHs) (fluorene, benzofluorene, pyrene, benzo(a)pyrene, and perylene) in dairy products using constant‐wavelength synchronous fluorescence spectrometry (CWSFS) was established in this study. Acetonitrile was chosen as the extraction solvent to extract the five PAHs from the dairy products, and an ultrasound extraction method was adopted. The supernatants were filtered using a 0.45‐μm microporous filter membrane and concentrated to dryness with a nitrogen dryer. The extracts were then re‐dissolved in cyclohexane for analysis. To overcome interference from the background matrix and between PAHs, the difference in wavelength (Δλ) at 40 nm was chosen for CWSFS scanning. With only one single scan, the five PAHs in dairy products could be distinguished and determined using the standard curve method without the need for previous chromatographic separation of the analyte solution. Detection limits of fluorene, benzofluorene, pyrene, benzo(a)pyrene, and perylene were 0.0051 μg·L⁻¹, 0.016 μg·L⁻¹, 0.021 μg·L⁻¹, 0.0056 μg·L⁻¹, and 0.0062 μg·L⁻¹, respectively. Recoveries were between 85.60% and 98.42%. These five PAHs in dairy products were determined with good results and therefore expected to be a routine detection method for PAHs in dairy products.

中文翻译：

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恒定波长同步荧光光谱法同时快速测定乳制品中的五个多环芳烃残基

同时测定五种多环芳烃（芴，苯并芴，pyr，苯并（a本研究建立了使用恒定波长同步荧光光谱法（CWSFS）测定乳制品中的py和and。选择乙腈作为提取溶剂从乳制品中提取五种多环芳烃，并采用超声提取的方法。上清液用0.45μm的微孔滤膜过滤，并用氮气干燥器浓缩至干。然后将提取物重新溶解在环己烷中进行分析。为了克服背景矩阵和PAH之间的干扰，选择40 nm处的波长差（Δλ）进行CWSFS扫描。只需进行一次扫描，就可以使用标准曲线方法区分和测定乳制品中的五种PAH，而无需事先对分析物溶液进行色谱分离。一个）芘和苝为0.0051微克·L ^-1，0.016微克·L ^-1，0.021微克·L ^-1，0.0056微克·L ^-1和0.0062微克·L ^-1，分别。回收率在85.60％至98.42％之间。乳制品中这五种多环芳烃的测定结果良好，因此有望成为乳制品中多环芳烃的常规检测方法。