In this study, two double-anode microbial fuel cells (MFCs) were connected in series for degradation of the azo dye reactive brilliant red X-3B. After the series connection, the electricity generation of one of the MFCs decreased, and the other was not affected too much. Due to the special structure in the double-anode MFC reduced the imbalanced performance between the MFC units, the occurrence of voltage reversal was limited. The removal efficiencies in two MFC reactors were not consistent after the series connection, the results showed that the MFC with the reduced electricity generation had the higher removal efficiencies, it was 12.90, 11.66, and 40.05% higher than in the MFC in which the power generation capacity was not affected after the series connection, the MFC without serial connection, and the control group, respectively. Meanwhile, the microbial communities related to the degradation of refractory organic compounds increased and related to electricity generation decreased in the MFC with the reduced electricity generation, the changes of the microbial communities were consistent with its electricity generation and the removal efficiencies. The degradation products in the effluent from two MFC units showed that had the products generated from the MFC with the reduced electricity generation had simpler structures comparing the other MFC unit.

在这项研究中，两个双阳极微生物燃料电池（MFCs）串联连接，以降解偶氮染料活性艳红X-3B。串联后，其中一个MFC的发电量减少，而另一个MFC的发电量没有受到太大影响。由于双阳极MFC的特殊结构减少了MFC单元之间的不平衡性能，因此限制了电压反转的发生。串联后，两个MFC反应器的去除效率不一致，结果表明，发电量降低的MFC的去除效率更高，分别比有功率的MFC高12.90、11.66和40.05％。串联连接，不带串行连接的MFC和对照组后，发电量均未受影响。与此同时，随着发电量的减少，MFC中与难降解有机物降解有关的微生物群落增加而与发电量相关的微生物群落减少，微生物群落的变化与其发电量和去除效率相吻合。来自两个MFC单元的废水中的降解产物表明，与另一个MFC单元相比，由MFC产生的产物具有降低的发电量，其结构更简单。