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Baicalein-modified hydroxyapatite nanoparticles and coatings with antibacterial and antioxidant properties
Biomaterials Advances ( IF 7.9 ) Pub Date : 2020-09-22 , DOI: 10.1016/j.msec.2020.111537
Estelle Palierse , Christophe Hélary , Jean-Marc Krafft , Isabelle Génois , Sylvie Masse , Guillaume Laurent , Maria I. Alvarez Echazu , Mohamed Selmane , Sandra Casale , Laetitia Valentin , Antoine Miche , Ben C.L. Chan , Clara B.S. Lau , Margaret Ip , Martin F. Desimone , Thibaud Coradin , Claude Jolivalt

Aseptic loosening and bacterial infections are the two main causes of failure for metallic implants used for joint replacement. A coating that is both bioactive and possesses antimicrobial properties may address such shortcomings and improve the performance of the implant. We have sought to study the properties of combining hydroxyapatite-based nanoparticles or coatings with baicalein, a plant-extracted molecule with both antibacterial and antioxidant properties. (B-type) carbonated hydroxyapatite nanoparticles prepared by a chemical wet method could subsequently adsorbed by soaking in a baicalein solution. The amount of adsorbed baicalein was determined to be 63 mg.g-1 by thermogravimetric measurements. In a second approach, baicalein was adsorbed on a biomimetic calcium-deficient hydroxyapatite planar coating (12 μm thick) deposited on Ti6Al4V alloy from an aqueous solution of calcium, phosphate, sodium and magnesium salts. Soaking of the hydroxyapatite coated on titanium alloy in a baicalein solution induced partial dissolution/remodeling of the upper surface of the coating. However, the observed remodeling of the surface was much more pronounced in the presence of a baicalein solution, compared to pure water. The presence of adsorbed baicalein on the HAp layer, although it could not be precisely quantified, was assessed by XPS and fluorescence analysis. Planar coatings exhibited significant antibacterial properties against Staphylococcus epidermidis. Baicalein-modified nanoparticles exhibited significant antioxidant properties. These results illustrate the potential of hydroxyapatite used as a carrier for natural biologically-active molecules and also discuss the challenges associated with their applications as antibacterial agents.



中文翻译:

黄ical素改性的羟基磷灰石纳米粒子和具有抗菌和抗氧化性能的涂料

无菌松动和细菌感染是用于关节置换的金属植入物失效的两个主要原因。具有生物活性并具有抗微生物特性的涂层可以解决这些缺点并改善植入物的性能。我们试图研究基于羟基磷灰石的纳米粒子或涂层与黄ical素(一种具有抗菌和抗氧化特性的植物提取分子)结合的特性。通过化学湿法制备的(B型)碳酸羟基磷灰石纳米颗粒随后可以通过浸入黄ical素溶液中进行吸附。黄ical素的吸附量确定为63 mg.g -1通过热重测量。在第二种方法中,将黄ical素从钙,磷酸盐,钠和镁盐的水溶液中吸附到仿生的缺钙钙磷灰石平面涂层(厚度为12μm)上,该涂层沉积在Ti6Al4V合金上。将涂覆在钛合金上的羟基磷灰石浸泡在黄ical素溶液中会引起涂层上表面的部分溶解/重塑。然而,与纯水相比,在黄ba素溶液存在下观察到的表面重塑更为明显。尽管无法精确定量,但仍通过XPS和荧光分析评估了HAp层上吸附的黄ical素的存在。平面涂料对表皮葡萄球菌具有显着的抗菌性能。黄ical素修饰的纳米颗粒表现出显着的抗氧化性能。这些结果说明了羟基磷灰石用作天然生物活性分子载体的潜力,并讨论了其作为抗菌剂的应用所面临的挑战。

更新日期:2020-09-22
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