The amine-bis(phenolate) ligands was prepared through a Mannich reaction utilizing two equivalents of 2,4-di-tert-butylphenol or 4-tert-butylphenol, two equivalents of formaldehyde and a single equivalent of 2-aminoethyl diphenylborinate as primary amine. This work deals with the synthesis and evaluation of new (B←N) and (B-O) units containing amine-bis(phenolate) boron complexes designed by combination of amine-bis(phenolate) ligands and various boronic acids in toluene reflux using a Dean-Stark apparatus to remove water formed as a by-product. The newly synthesized amine-bis(phenolate) ligands and their boron complexes were characterized using elemental analysis, and their probable structures were proposed based on ¹H and ¹³C NMR, FT-IR, UV-Vis spectroscopy and LC-MS/MS spectrometry. The inhibition effects of the synthesized compounds on acetylcholinesterase (AChE) activity in vitro and their radical scavenging activities were evaluated. The AChE was effectively inhibited by compounds, with K_I values in the range from 5.48 ± 0.65 to 40.56 ± 4.42 µM. The (L₁B₄) has more inhibition effect on AChE with K_I value (6.37 ± 1.50 µM) in (L₂) series, while (L₂B₄) has an inhibition effect with K_I (5.48 ± 0.65 µM) in (L₂) series. Also, the compounds showed about 18-45% DPPH and 26-85% ABTS radical scavenging activity. On the other hand, butylated hydroxy anisole (BHA), butylated hydroxytoluene (BHT) and Trolox showed about 72-96% DPPH and 94-96% ABTS radical scavenging activity, respectively in the same concentration (25-30 µg/mL). Besides, the (L₁B₄), (L₁B₅), and (L₂B₄) determined as the most active inhibitors were docked into the binding site of AChE to find the binding interactions of the boron complexes with the protein.

通过曼尼希反应，使用两当量的2,4-二叔丁基苯酚或4-叔丁基苯酚，两当量的甲醛和一当量的2-氨基乙基二苯基硼酸酯作为伯胺，通过曼尼希反应制备胺-双（酚盐）配体。这项工作涉及合成和评估含有胺-双（酚盐）硼配合物的新（B←N）和（B - O）单元，该配合物是通过胺-双（酚盐）配体和各种硼酸在甲苯回流中的组合而设计的。 Dean-Stark装置，用于去除副产品中形成的水​​。用元素分析法对新合成的胺-双（酚盐）配体及其硼配合物进行了表征，并基于¹ H和¹ H提出了可能的结构。¹³ C NMR，FT-IR，UV-Vis光谱和LC-MS / MS光谱。评价了合成化合物对乙酰胆碱酯酶（AChE）的体外抑制作用及其自由基清除活性。AChE被化合物有效抑制，K _I值在5.48±0.65至40.56±4.42 µM的范围内。的（L ₁乙₄）具有乙酰胆碱酯酶抑制更效果ķ_我值（6.37±1.50μM）在（L ₂）系列，而（L ₂乙₄）具有带有抑制作用ķ_我（L ₂）系列中的（5.48±0.65 µM）。而且，该化合物显示约18-45％的DPPH和26-85％的ABTS自由基清除活性。另一方面，在相同浓度（25-30 µg / mL）下，丁基化羟基茴香醚（BHA），丁基化羟基甲苯（BHT）和Trolox分别显示出约72-96％的DPPH和94-96％的ABTS自由基清除活性。此外，将被确定为活性最高的抑制剂（L ₁ B ₄），（L ₁ B ₅）和（L ₂ B ₄）停泊在AChE的结合位点，以发现硼配合物与蛋白质的结合相互作用。 。