Abstract Recently, the composites of varying biomaterials and two-dimensional (2D) nanomaterials have been increasingly developed in the field of tissue engineering and regeneration owing to their excellent physicochemical and mechanical properties. In particular, the combination of biocompatible polymers and 2D nanomaterials can endow the nanocomposite with novel biofunctional properties while maintaining the inherent character of every single material. These nanocomposites are known to have the potential for improvement of cellular behaviors such as cell adhesion, proliferation, and differentiation. In this study, poly(l-lactide-co-e-caprolactone) (PLCL) and laminin (Lam) nanofibers functionalized with black phosphorus (BP) were successfully fabricated to increase the neuritogenesis of HT22 hippocampal neuronal cells using an electrospinning process. The ternary nanofiber matrices of PLCL/Lam/BP were found to be suitable for supporting the attachment and proliferation of hippocampal neurons. Furthermore, these PLCL/Lam/BP nanofiber matrices could not only promote neurite outgrowth and alignment but also increase the neuritogenesis of hippocampal neurons by providing optimal microenvironments for neuronal differentiation. The underlying mechanism of this phenomenon was confirmed by determining the expression levels of genes related to neurogenesis. In conclusion, these results suggest that BP-functionalized composite nanofibers could be a promising candidate as a scaffold for neural tissue engineering and regeneration.

中文翻译：

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用黑磷装饰的 PLCL 和层粘连蛋白的三元纳米纤维基质上的神经发生增加

摘要 近年来，各种生物材料和二维（2D）纳米材料的复合材料由于其优异的物理化学和机械性能，在组织工程和再生领域得到了越来越多的发展。特别是，生物相容性聚合物和二维纳米材料的结合可以赋予纳米复合材料新的生物功能特性，同时保持每种材料的固有特性。已知这些纳米复合材料具有改善细胞行为如细胞粘附、增殖和分化的潜力。在这项研究中，聚（l-丙交酯-共-ε-己内酯）（PLCL）和用黑磷（BP）功能化的层粘连蛋白（Lam）纳米纤维被成功制造，以使用静电纺丝工艺增加 HT22 海马神经元细胞的神经发生。发现 PLCL/Lam/BP 的三元纳米纤维基质适合支持海马神经元的附着和增殖。此外，这些 PLCL/Lam/BP 纳米纤维基质不仅可以促进神经突生长和排列，还可以通过为神经元分化提供最佳微环境来增加海马神经元的神经发生。通过确定与神经发生相关的基因的表达水平，证实了这种现象的潜在机制。综上所述，