Low pH and high salinity characteristic of produced water (PW) posed a big challenge for the direct biological treatment. The immobilization of R. qingshengii strain FF, which degraded petroleum effectively under low pH, and application of immobilized R. qingshengii strain FF in treating mimic PW was studied in this work. The immobilization of R. qingshengii strain FF on the surface of polyethylene foam (PEF), one type of waste packaging materials, was optimized using the response surface methodology. Under optimum conditions, cell density of R. qingshengii strain FF immobilized on the surface of PEF reached 388 mg (cells)/g(PEF). In addition, a few factors, including hydraulic retention time (HRT), pH and salinity, were studied for treating mimic PW using immobilized R. qingshengii strain FF. The result of this study demonstrated that TPH degradation efficiency of PW by immobilized R. qingshengii strain FF reached above 90% when HRT was longer than 8 h. Weak acid and high salinity conditions only moderately decreased TPH. Asphalt, alkanes and aromatic hydrocarbon contained in petroleum can be degraded to some extent. These results indicated that immobilized R. qingshengii strain FF can be used as a highly efficient strain which could be used in biological treatment of real PW.

采出水（PW）的低pH和高盐度特性对直接生物处理提出了巨大挑战。的固定化R. qingshengii应变FF，其低pH下有效降解石油，和固定化的应用R. qingshengii治疗模拟PW应变FF在这项工作中进行了研究。使用响应表面方法优化了青生李斯特菌菌株FF在聚乙烯泡沫（PEF）（一种废物包装材料）表面的固定化。在最佳条件下，青生罗非鱼的细胞密度固定在PEF表面的FF菌株达到388mg（细胞）/ g（PEF）。此外，还研究了使用固定化青生李氏杆菌FF处理水力停留时间（HRT），pH值和盐度等一些因素来处理模拟PW的方法。这项研究的结果表明，当HRT超过8 h时，固定化的青枯菌FF对PW的TPH降解效率达到90％以上。弱酸和高盐度条件只能适度降低TPH。石油中包含的沥青，烷烃和芳烃可以在一定程度上降解。这些结果表明，固定化的青枯菌FF可以用作高效菌株，可以用于实际PW的生物处理。