In this study, RNA sequencing was used to identify the hepatic gene expression profile in grass carp associated with luteinizing hormone-releasing hormone agonist (LHRH-A) treatment. A total of 93,912,172 reads were generated by HiSeq 4000 sequencing platform. After filtering, 83,450,860 clean reads were mapped to the reference genome. By calculating the FPKM of genes, 1475 differentially expressed genes were identified. PPAR signaling pathway was enriched with upregulated genes in LHRH-A injection group showing the regulation of the lipid metabolism by LHRH-A. The expression of eight key genes in PPAR signaling pathway was confirmed by qPCR and the results suggested that ACSL4A, ACSL4B, ANGPTL4, LPL, RXRBA and SLC27A1B were significantly stimulated by LHRH-A injection. This investigation provides the evidence that LHRH-A could play a role in lipid metabolism.

在本研究中，RNA 测序用于鉴定草鱼中与促黄体激素释放激素激动剂 (LHRH-A) 治疗相关的肝脏基因表达谱。HiSeq 4000 测序平台总共生成了 93,912,172 条读数。过滤后，83,450,860 个干净的读数被映射到参考基因组。通过计算基因的FPKM，鉴定出1475个差异表达基因。LHRH-A注射组PPAR信号通路富含上调基因，显示LHRH-A对脂质代谢的调节作用。通过qPCR证实了PPAR信号通路中8个关键基因的表达，结果表明ACSL4A、ACSL4B、ANGPTL4、LPL、RXRBA和SLC27A1B受到LHRH-A注射的显着刺激。