The association between Coronary Artery Calcification (CAC) and osteoporosis has been reported but not fully understood. Therefore, using an original bioinformatic framework we analyzed transcriptomic profiles of 20 elderly women with high CAC score and 31 age- and sex-matching controls from São Paulo Aging & Health study. We integrated differentially expressed microRNA (miRNA) and long-noncoding RNA (lncRNA) interactions with coding genes associated with CAC, in the context of bone-metabolism genes mined from literature. Top non-coding regulators of bone metabolism in CAC included miRNA 497-5p/195 and 106a-5p, and lncRNA FAM197Y7. Top non-coding RNAs revealed significant interplay between genes regulating bone metabolism, vascularization-related processes, chromatin organization, prostaglandin and calcium co-signaling. Prostaglandin E2 receptor 3 (PTGER3), Fibroblasts Growth Factor Receptor 1 (FGFR1), and One Cut Homeobox 2 (ONECUT2) were identified as the most susceptible to regulation by the top non-coding RNAs. This study provides a flexible transcriptomic framework including non-coding regulation for biomarker-related studies.

冠状动脉钙化 (CAC) 与骨质疏松症之间的关联已有报道，但尚未完全了解。因此，使用原始生物信息学框架，我们分析了来自圣保罗老龄化与健康研究的 20 名 CAC 评分高的老年女性和 31 名年龄和性别匹配对照的转录组谱。在从文献中挖掘的骨代谢基因的背景下，我们整合了差异表达的 microRNA (miRNA) 和长链非编码 RNA (lncRNA) 与与 CAC 相关的编码基因的相互作用。CAC 中骨代谢的顶级非编码调节因子包括 miRNA 497-5p/195 和 106a-5p，以及 lncRNA FAM197Y7。顶级非编码 RNA 揭示了调节骨代谢、血管化相关过程、染色质组织、前列腺素和钙协同信号传导的基因之间的显着相互作用。前列腺素 E2 受体 3 (PTGER3)、成纤维细胞生长因子受体 1 (FGFR1) 和 One Cut Homeobox 2 (ONECUT2) 被确定为最容易受到顶级非编码 RNA 调节的影响。本研究提供了一个灵活的转录组学框架，包括生物标志物相关研究的非编码调控。