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Characterisation of IL-15 and IL-2Rβ in grass carp: IL-15 upregulates cytokines and transcription factors of type 1 immune response and NK cell activation
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-09-22 , DOI: 10.1016/j.fsi.2020.09.029
Wei Wang , Junya Wang , Lina Lei , Jiawen Xu , Yuting Qin , Qian Gao , Jun Zou

Interleukin (IL) −15 belongs to the common cytokine receptor γ chain (γC) family and has diverse functions in regulating the development, proliferation and activation of NK and T cells. It activates a hetero-trimeric receptor complex consisting of IL-2Rα, IL-2Rβ and a common γ chain (γC). In this study, the full-length cDNA sequences of IL-15 and IL-2Rβ were identified in grass carp (Ctenopharyngodon idella, Ci) and their expression profiles analysed. The CiIL-15 and CiIL-2Rβ were shown to be broadly expressed in tissues, with the highest levels detected in the spleen. Moreover, the CiIL-15 and CiIL-2Rβ were modulated in primary head kidney leucocytes (HKLs) and splenocytes by immunostimulants and cytokines, and in the head kidney and spleen of fish after infection of Flavobacterium columnare and grass carp reovirus. The bioactivity of bacteria derived recombinant CiIL-15 protein was evaluated in the primary leucocytes. The CiIL-15 was shown to induce signature genes of type 1 immune response (IFN-γ and T-bet) and NK cell activation (perforin and Eomesa), whilst exhibiting inhibitory effects on the genes involved in the type 2 immune response (IL-4/13, IL-10 and Gata3). Our data suggest that IL-15 is a key regulator in promoting the type 1 immune response and NK cell activation in fish.



中文翻译:

草鱼中IL-15和IL-2Rβ的特征:IL-15上调1型免疫应答和NK细胞活化的细胞因子和转录因子

白介素(IL)-15属于常见的细胞因子受体γ链(γC)家族,在调节NK和T细胞的发育,增殖和活化中具有多种功能。它激活由IL-2Rα,IL-2Rβ和一条共同的γ链(γC)组成的异三聚体受体复合物。在这项研究中,在草鱼(Ctenopharyngodon idella,Ci)中鉴定了IL-15和IL-2Rβ的全长cDNA序列,并分析了它们的表达谱。显示Ci IL-15和CiIL -2Rβ在组织中广泛表达,在脾脏中检测到最高水平。此外,Ci IL-15和Ci感染黄杆菌和草鱼呼肠孤病毒后,免疫刺激剂和细胞因子可调节原发性头部肾脏白细胞(HKLs)和脾细胞中的IL-2Rβ,以及鱼类头部肾脏和脾脏中的IL-2Rβ 。在原代白细胞中评估了细菌衍生的重组Ci IL-15蛋白的生物活性。所述IL-15显示出诱导1型免疫应答(IFN-γ和T-BET)和NK细胞活化(穿孔和Eomesa)的签名基因,同时表现出对参与的2型免疫应答的基因抑制效应( IL-4 / 13,IL-10和Gata3)。我们的数据表明,IL-15是促进鱼类1型免疫应答和NK细胞活化的关键调节剂。

更新日期:2020-10-15
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