β-1,3-xylanase plays an important role in the conversion of algal biomass into renewable chemical commodities and functional xylooligosaccharides. Efficient immobilization of the target enzymes that could integrate the separation, purification, and immobilization in one-step may have great potentials. The cheap and easily obtained pitch was adopted as the raw material to prepare the hyper-cross-linked polymers with the specific surface areas of 560 m2/g and the Ni2+ loading about 80 mg/g. As a carrier, it could immobilize and purify the β-1,3-xylanases directly from the crude cell debris with the activity recovery of (80.66 ± 0.87) %. The half-life of the immobilized β-1,3-xylanases was 50 min at 50 °C, which was significantly longer than the free ones with only 14 min. The immobilized β-1,3-xylanases could retain 65 % of the activity after stored at 30 ℃ for 80 h, they could also retain 87 % of the original activity after 15 cycles of reuse. In terms of potential applications in the manipulation of algae Caulerpa lentillifera, the immobilized β-1,3-xylanases could release 30 % more reducing sugar in 85 h. The method proposed here is promising for algal biomass manipulation because of its low cost, stable storage, convenient recycling and excellent reusability.

中文翻译：

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将 β-1,3-木聚糖酶固定在负载有 Ni2+ 的沥青基超交联聚合物上用于藻类生物质操作

β-1,3-木聚糖酶在将藻类生物质转化为可再生化学商品和功能性低聚木糖方面发挥着重要作用。能够将分离、纯化和固定化一体化的目标酶的高效固定化可能具有巨大的潜力。采用廉价易得的沥青为原料制备比表面积为560 m2/g、Ni2+负载量约为80 mg/g的超交联聚合物。作为载体，它可以直接从粗细胞碎片中固定和纯化β-1,3-木聚糖酶，活性恢复率为（80.66±0.87）%。固定化 β-1,3-木聚糖酶在 50°C 下的半衰期为 50 分钟，明显长于仅 14 分钟的游离酶。固定化的β-1，3-木聚糖酶在30 ℃保存80 h后仍可保持65%的活性，重复使用15次后仍可保持87%的原活性。在操纵藻类 Caulerpa lentillifera 的潜在应用方面，固定化的 β-1,3-木聚糖酶可以在 85 小时内多释放 30% 的还原糖。这里提出的方法由于成本低、储存稳定、回收方便和良好的可重复使用性而有望用于藻类生物质处理。