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C-terminally encoded peptides (CEPs) are potential mediators of abiotic stress response in plants
Physiology and Molecular Biology of Plants ( IF 3.5 ) Pub Date : 2020-09-21 , DOI: 10.1007/s12298-020-00881-4
Sakshi Aggarwal 1 , Ashish Kumar 1 , Muskan Jain 1 , Jebi Sudan 2 , Kapil Singh 1 , Sumita Kumari 2 , Ananda Mustafiz 1
Affiliation  

Intracellular signaling is a critical determinant of the normal growth and development of plants. Signaling peptides, also known as peptide hormones, along with classical phytohormones, are the significant players of plant intracellular signaling. C-terminally encoded peptide (CEP), a 15-amino acid post-translationally peptide identified in Arabidopsis, plays a pivotal role in lateral root formation, nodulation, and act as long-distance root to shoot signaling molecule in N-starvation conditions. Expression of CEP gene members in Arabidopsis is perturbed by nitrogen starvation; however, not much is known regarding their role in other abiotic stress conditions. To gain a comprehensive insight into CEP biology, we identified CEP genes across diverse plant genera (Glycine max, Sorghum bicolor, Brassica rapa, Zea mays, and Oryza sativa) using bioinformatics tools. In silico promoter analysis revealed that CEP gene promoters show an abundance of abiotic stress-responsive elements suggesting a possible role of CEPs in abiotic stress signaling. Spatial and temporal expression patterns of CEP via RNA seq and microarray revealed that various CEP genes are transcriptionally regulated in response to abiotic stresses. Validation of rice CEP genes expression by qRT-PCR showed that OsCEP1, OsCEP8, OsCEP9, and OsCEP10 were highly upregulated in response to different abiotic stress conditions. Our findings suggest these CEP genes might be important mediators of the abiotic stress response and warrant further overexpression/knockout studies to delineate their precise role in abiotic stress response.



中文翻译:

C-末端编码肽(CEPs)是植物非生物胁迫反应的潜在介质

细胞内信号传导是植物正常生长和发育的关键决定因素。信号肽,也称为肽激素,与经典植物激素一起,是植物细胞内信号传导的重要参与者。C-末端编码肽 (CEP) 是一种在拟南芥中鉴定的 15 个氨基酸的翻译后肽,在侧根形成、结瘤中起着关键作用,并在 N 饥饿条件下作为长距离根到芽的信号分子。拟南芥中 CEP 基因成员的表达受到氮饥饿的干扰;然而,关于它们在其他非生物胁迫条件下的作用知之甚少。为了全面了解 CEP 生物学,我们鉴定了不同植物属(Glycine maxSorghum bicolor芸苔玉米水稻)使用生物信息学工具。计算机启动子分析显示,CEP 基因启动子显示出丰富的非生物胁迫响应元件,表明 CEP 在非生物胁迫信号传导中可能发挥作用。通过 RNA seq 和微阵列的 CEP 时空表达模式表明,各种 CEP 基因在响应非生物胁迫时受到转录调控。通过 qRT-PCR 验证水稻 CEP 基因表达表明OsCEP1OsCEP8OsCEP9OsCEP10响应不同的非生物胁迫条件而高度上调。我们的研究结果表明,这些 CEP 基因可能是非生物胁迫反应的重要介质,需要进一步的过度表达/敲除研究来描述它们在非生物胁迫反应中的确切作用。

更新日期:2020-09-22
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