Purpose

To visualize SARS-CoV-2 host receptors ACE2 and CD147 on human oocytes and blastocysts.

Methods

Immunohistochemistry and confocal microscopy on human primary oocytes and pre (5 days post fertilization (dpf5) and (dpf6))- and peri (dpf7)-implantation blastocysts donated to research.

Results

SARS-CoV-2 host receptors ACE2 and CD147 are present on the membrane of trophectoderm, epiblast and hypoblast cells in human blastocysts. CD147 is also present on the oolemma.

Conclusion

Theoretically, the earliest stages of embryonic development may be vulnerable for SARS-CoV-2 infection.

中文翻译：

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SARS-CoV-2宿主受体ACE2和CD147（BSG）存在于人卵母细胞和胚泡中。

目的

为了可视化人类卵母细胞和胚泡上的SARS-CoV-2宿主受体ACE2和CD147。

方法

免疫组织化学和共聚焦显微镜在人类原代卵母细胞和受精前（受精后5天（dpf5）和（dpf6））和植入前（dpf7）胚泡的研究中获得了成功。

结果

SARS-CoV-2宿主受体ACE2和CD147存在于人胚泡的滋养外胚层，上皮细胞和下胚层细胞的膜上。CD147也存在于血肿中。

结论

从理论上讲，最早的胚胎发育阶段可能容易感染SARS-CoV-2。