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Molecular diversity analysis of genotypes from four Aegilops species based on retrotransposon–microsatellite amplified polymorphism (REMAP) markers
Cereal Research Communications ( IF 1.6 ) Pub Date : 2020-09-21 , DOI: 10.1007/s42976-020-00086-1
Justyna Leśniowska-Nowak , Sylwia Okoń , Aleksandra Wieremczuk

Genetic diversity analysis is an important tool in crop improvement. Species with high genetic diversity are a valuable source of variation used in breeding programs. The aim of this study was to assess the genetic diversity of four species belonging to the genus Aegilops, which are often used to expand the genetic variability of wheat and triticale. Forty-five genotypes belonging to the genus Aegilops were investigated. Within- and among-species genetic diversity was calculated based on REMAP (retrotransposon–microsatellite amplified polymorphism) molecular markers. Obtained results showed that REMAP markers are a powerful method for genetic diversity analysis, which produces a high number of polymorphic bands (96.09% of total bands were polymorphic). Among tested genotypes, Ae. crassa and Ae. vavilovii showed the highest genetic diversity and should be chosen as a valuable source of genetic variation.

中文翻译:

基于逆转录转座子-微卫星扩增多态性 (REMAP) 标记的四种 Aegilops 物种基因型的分子多样性分析

遗传多样性分析是作物改良的重要工具。具有高遗传多样性的物种是育种计划中使用的宝贵变异来源。本研究的目的是评估属于 Aegilops 属的四个物种的遗传多样性,这些物种通常用于扩大小麦和小黑麦的遗传变异性。研究了属于 Aegilops 属的 45 种基因型。基于REMAP(逆转录转座子-微卫星扩增多态性)分子标记计算种内和种间遗传多样性。获得的结果表明,REMAP 标记是一种强大的遗传多样性分析方法,它产生了大量的多态条带(96.09% 的条带是多态的)。在测试的基因型中,Ae。克拉萨和 Ae。
更新日期:2020-09-21
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