This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, including Marinobacter hydrocarbonoclaustics and Marinobacter alkaliphilus. Electrochemical analysis such as impedance and polarization results indicated that Proteobacteria biofilm accelerated corrosion by ~ twofold (2.1 ± 0.61 mm/years) or ~ fourfold (~ 3.7 ± 0.42 mm/years) when compared to the control treatment (0.95 ± 0.1 mm/years). Scanning electron and atomic force microscopy revealed the presence of a thick biofilm and pitting corrosion. X-ray diffraction revealed higher amounts of the corrosion products Fe₂O₃, γ-FeOOH, and α-FeOOH, and confirmed that the microbial biofilm strongly oxidized the iron and contributed to the acceleration of corrosion of carbon metal API 5LX.

本研究旨在表征导致 API 5LX 碳钢腐蚀的生物膜微生物群落。API 5LX 碳钢试样与从两个油藏站收集的原采出水或过滤消毒的采出水一起孵育。生物膜 16S rRNA 扩增子测序显示，生物膜中存在的细菌群落以变形菌为主，包括Marinobacter carbonoclaustics和Marinobacter basephilus. 电化学分析，如阻抗和极化结果表明，与对照处理（0.95±0.1 毫米/年）相比，变形杆菌生物膜将腐蚀加速了~两倍（2.1±0.61 毫米/年）或~四倍（~3.7±0.42 毫米/年） ）。扫描电子和原子力显微镜显示存在厚厚的生物膜和点蚀。X 射线衍射显示腐蚀产物 Fe ₂ O ₃、γ-FeOOH 和 α-FeO​​OH 的含量更高，并证实微生物生物膜强烈氧化铁并有助于加速碳金属 API 5LX 的腐蚀。