Spinal cord injury includes inflammation and apoptosis of neurons, which is difficult to cure by systemic drug administration. Administration of natural active compounds (resveratrol and also Puerarin) by advance drug delivery technology improves the patient’s conditions. Oil-in-water emulsion method was utilized to prepare resveratrol as well as puerarin loaded PLGA nanoparticles. The nanoparticles were subjected to mean zeta potential, mean particle size, encapsulation efficiency as well as in vitro drug release studies. The biochemical parameters i.e. malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), advanced oxidation products (AOPP), catalase (CAT) and nitrite/nitrate levels were tested for the loaded nanoparticles. Reperfusion injury induced rats treated with 10 mg/kg resveratrol and puerarin loaded nanoparticles protects spine from ischemia injury and supports biological parameters. The mean particle size varies from 238 nm to 274 nm and also particle size distribution was mono-dispersed (0.239 to 0.318). Zeta potential value of nanoparticles was observed to be −12.6 ± 2.1 mV. Optimized nanoparticles reveals 72% -79% of drug release over 36 h by diffusion mechanism. Significantly, lowers the levels of plasma nitrite/nitrate level as well as phosphorylation of p38MAPK pathways in reperfusion injury induced rats. The resveratrol and puerarin loaded nanoparticles decreases free radicals produced by reperfusion injury induced rats, as well as decrease of oxidative stress because of IRI. Resveratrol and puerarin loaded nanoparticles decreases GSH, SOD and CAT antioxidant level, which helps in overall health improvement of patients.

脊髓损伤包括神经元的炎症和凋亡，全身用药难以治愈。通过先进的药物输送技术施用天然活性化合物（白藜芦醇和葛根素）可改善患者的状况。采用水包油乳液法制备白藜芦醇和葛根素负载的PLGA纳米颗粒。对纳米颗粒进行平均 zeta 电位、平均粒径、封装效率以及体外药物释放研究。生化参数，即丙二醛 (MDA)、还原型谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD)、高级氧化产物 (AOPP)、过氧化氢酶 (CAT) 和亚硝酸盐/硝酸盐水平对负载的纳米颗粒进行测试。用 10 mg/kg 白藜芦醇和葛根素纳米颗粒处理再灌注损伤诱导的大鼠可保护脊柱免受缺血损伤并支持生物学参数。平均粒度从 238 nm 到 274 nm 不等，而且粒度分布也是单分散的（0.239 到 0.318）。观察到纳米颗粒的 Zeta 电位值为 -12.6 ± 2.1 mV。优化的纳米粒子通过扩散机制在 36 小时内显示 72% -79% 的药物释放。显着降低再灌注损伤诱导大鼠的血浆亚硝酸盐/硝酸盐水平以及 p38MAPK 通路的磷酸化。负载白藜芦醇和葛根素的纳米颗粒减少再灌注损伤诱导的大鼠产生的自由基，以及减少因 IRI 引起的氧化应激。负载白藜芦醇和葛根素的纳米颗粒降低 GSH，