Obstacles in different tissue layers of leaves can influence aphid feeding behavior and host preference. In this study, we examined the feeding behaviors of cabbage aphids Brevicoryne brassicae (Linnaeus) (Hemiptera: Aphididae) on the five oilseed rape cultivars with varied levels of aphid susceptibility using the electropenetrography (EPG) technique. The leaf anatomical structures of the five oilseed rape cultivars were also analyzed using the microscopical observations. The results showed that the cabbage aphid feeding behaviors varied among the five cultivars. On ‘Dehezayou8’ which had the highest aphid population in the field, the cabbage aphids showed significantly shorter non-probing time to the first phloem contact, shorter duration of the no phloematic phase, and longer average probing duration than those on ‘Zhongyou821’ which showed the least aphid population. In the mesophyll of ‘Dehezayou8,’ the cabbage aphids made significantly fewer brief probes and spent a shorter time in penetrating the mesophyll cells than on ‘Zhongyou821.’ In the phloem, the aphids on ‘Dehezayou8’ secreted less saliva and ingested more sap than those on ‘Zhongyou821.’ On ‘Dehezayou8,’ the aphids took the shortest time to first penetration than they did on the other cultivars. ‘Dehezayou8’ had relatively thin upper epidermis and rare short trichomes, which might have facilitated the aphid infestation. Our results suggested that the leaf tissue characteristics of oilseed rape cultivars, such as epidermis thickness, trichomes, and the factors from mesophyll and phloem could be associated with aphid feeding preference and therefore aphid population in the field.

叶片不同组织层中的障碍物会影响蚜虫的进食行为和寄主的偏好。在这项研究中，我们研究了白菜蚜Brevicoryne brasicae的摄食行为（Linnaeus）（Hemiptera：Aphididae）在五个油菜油菜品种上使用电渗透法（EPG）技术检测了不同水平的蚜虫敏感性。还使用显微镜观察结果分析了五个油料油菜品种的叶片解剖结构。结果表明，五个品种的白菜蚜虫取食行为各不相同。在田间蚜虫数量最多的“ Dehezayou8”上，与“ Zhongyou821”相比，卷心菜蚜虫对首次韧皮部接触的非探测时间明显缩短，无发色期的持续时间较短，平均探测时间更长。蚜虫数量最少。在'Dehezayou8'的叶肉中 与“中油821”相比，白菜蚜虫进行的短暂探针明显少得多，并且穿透叶肉细胞的时间也更短。在韧皮部中，“中和油8”上的蚜虫比“中油821”上的唾液分泌更少的唾液并吸收了更多的汁液。与其他品种相比，在“ Dehezayou8”上，蚜虫第一次穿透的时间最短。'Dehezayou8'具有相对较薄的上表皮和罕见的短毛状体，这可能促进了蚜虫的侵染。我们的研究结果表明，油料油菜品种的叶片组织特性，例如表皮厚度，毛状体以及叶肉和韧皮部的因素，可能与蚜虫的摄食偏好有关，因此也与田间蚜虫种群有关。与“中油821”相比，“德和油8”的蚜虫分泌的唾液少，汁液摄入多。与其他品种相比，在“ Dehezayou8”上，蚜虫第一次穿透的时间最短。'Dehezayou8'具有相对较薄的上表皮和罕见的短毛状体，这可能促进了蚜虫的侵染。我们的研究结果表明，油料油菜品种的叶片组织特性，例如表皮厚度，毛状体以及叶肉和韧皮部的因素可能与蚜虫的取食偏好有关，因此也与田间蚜虫种群有关。与“中油821”相比，“德和油8”的蚜虫分泌的唾液少，汁液摄入多。与其他品种相比，在“ Dehezayou8”上，蚜虫第一次穿透的时间最短。'Dehezayou8'具有相对较薄的上表皮和罕见的短毛状体，这可能促进了蚜虫的侵染。我们的研究结果表明，油料油菜品种的叶片组织特性，例如表皮厚度，毛状体以及叶肉和韧皮部的因素可能与蚜虫的取食偏好有关，因此也与田间蚜虫种群有关。