The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.

中文翻译：

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基于荧光的体内流式细胞术的最新进展

基于荧光的体内流式细胞术 (IVFC) 是监测体内循环细胞的新兴工具。作为一种无创和实时诊断技术，基于荧光的 IVFC 可以在不改变其原生生物环境的情况下对循环细胞进行长期监测。它已被应用于各种生物学应用（例如，监测循环肿瘤细胞）。在这项工作中，我们将回顾我们最近关于基于荧光的 IVFC 的工作。将介绍其工作原理和典型的生物学应用。此外，还总结了基于光声效应的IVFC流式细胞术以及基于成像的方法、漫射光方法、混合多模态方法和多光谱方法等其他无标记检测方法的最新进展。