Restriction site-associated DNA sequencing allows for the rapid identification of simple sequence repeat markers in Echinochloa crus-galli,Weed Biology and Management

当前位置： X-MOL 学术 › Weed Biol. Manag. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Restriction site-associated DNA sequencing allows for the rapid identification of simple sequence repeat markers in Echinochloa crus-galli
Weed Biology and Management ( IF 1.4 ) Pub Date : 2017-04-27 , DOI: 10.1111/wbm.12119
Guoqi Chen _{1,

2} , Wei Zhang _{1,

2} , Jiangpeng Fang _{1,

2} , Liyao Dong _{1,

2}

Affiliation

Echinochloa crus-galli is a serious weed worldwide. Microsatellite markers (simple sequence repeats, SSRs) are important molecular markers that are used widely for studying genetic diversity in plants. However, a limited number of SSRs is available for E. crus-galli. The restriction site-associated DNA (RAD) sequencing approach was combined with Illumina DNA sequencing for the rapid and mass detection of SSRs in E. crus-galli. The RAD tags were generated from the genomic DNA of E. crus-galli and were sequenced in order to produce 6921.6 Mb of high-quality sequences with 45.1% guanine–cytosine content. In total, 3081 putative SSRs were detected, of which 82.2% were dinucleotide motif-repeats. AT was the most frequent motif, accounting for 35.0% of the SSRs. In order to test the validity of the SSRs that were developed here, eight SSRs that were selected from putative SSRs were used to study the genetic diversity and structures of 20 E. crus-galli populations that had been collected from rice fields in eastern China. Ninety-seven alleles were amplified from the eight microsatellite loci among the 20 E. crus-galli populations. These populations showed low genetic diversity and were classified on the basis of their genetic structures into three distinct groups that corresponded to the three regions of population sampling. The SSRs that were identified in this study represent a valuable resource for studying the genetic diversity, population biology and evolution of E. crus-galli.

更新日期：2017-04-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>