当前位置: X-MOL 学术Indian J. Biochem. Biophys. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Detection of anti-Mycobacterium avium subspecies paratuberculosis antibodies in thyroid and type-1 diabetes patients
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2020-09-21
Kundan Kumar Chaubey, Shoor Vir Singh, Pravin Kumar Singh, Saurabh Gupta, Vishal Khandelwal, Pradeep Kumar Choudhary, Gaurav Pant, Sujata Jayaraman, Krishna Dutta Rawat

Mycobacterium avium subspecies paratuberculosis (MAP) causes granulomatous intestinal disease in animals (Johne’s diseases). MAP has also been associated with several autoimmune disorders. In this study, we screened serum samples from confirmed patients of thyroid and type 1 diabetes for the presence of antibody against MAP. We used newly developed 'cocktail ELISA' (based on recombinant secretary proteins) and extensively validated 'indigenous ELISA' (based on whole cell protoplasmic antigen) and both the tests were also compared for their diagnostic potential. A total of 90 serums samples were included of which anti-MAP antibodies was detected in 28.8% and 26.6% of samples by indigenous ELISA (iELISA) and cocktail ELISA (cELISA), respectively. There was almost perfect agreement between the two tests in detecting the anti-MAP antibodies. Study raises concern on high detection of anti-MAP antibodies in human, thus warranting necessary control measure to minimize MAP exposure in human beings.

中文翻译:

甲状腺和1型糖尿病患者抗鸟分枝杆菌亚种副结核抗体的检测

鸟分枝杆菌亚种副结核病(MAP)会引起动物肉芽肿性肠病(约翰氏病)。MAP还与几种自身免疫性疾病有关。在这项研究中,我们从确诊的甲状腺和1型糖尿病患者的血清样本中筛选了针对MAP的抗体。我们使用了新开发的“鸡尾酒ELISA”(基于重组​​秘书蛋白)和经过广泛验证的“本地ELISA”(基于全细胞原生质抗原),并且还比较了这两种测试的诊断潜力。总共包括90个血清样品,其中通过本地ELISA(iELISA)和鸡尾酒ELISA(cELISA)分别检测到28.8%和26.6%的抗MAP抗体。两种测试在检测抗MAP抗体方面几乎完全吻合。
更新日期:2020-09-21
down
wechat
bug