Urease producing bacteria have been reported from various ecosystems including soil, water and host organisms such as humans and animals. This research describes the isolation and identification of ureolytic bacteria from calcium rich marine sediments of southern India. The potential strain was designated as UR1 and identified using polyphasic taxonomy. The results show that the strain belongs to the genus Virgibacillus and is closely related to the genus Bacillus. Further, the urease was extracted by 80% acetone precipitation method and the molecular weight was determined as ~80 kDA by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The high performance liquid chromatography (HPLC) analysis of the urease in comparison with standard indicates that the isolated enzyme is urease. The enzyme exhibited the maximum activity at the temperature of 45^oC and pH of 9. Furthermore, the crude enzyme exhibited notable calcite precipitation signifying that the strain could be used for microbial induced calcite precipitation (MICP) and subsequently for biocementation process.

中文翻译：

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鉴定产生脲酶的Virgibacillus sp.。海洋沉积物中的UR1

已经从各种生态系统，包括土壤，水和宿主生物（例如人和动物）中报告了产生脲酶的细菌。这项研究描述了从印度南部富含钙的海洋沉积物中分离和鉴定尿素分解细菌。潜在菌株命名为UR1，并使用多相分类法进行鉴定。结果表明该菌株属于病毒杆菌属，与芽孢杆菌属密切相关。此外，通过80％丙酮沉淀法提取脲酶，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）将分子量确定为〜80 kDA。与标准品相比，尿素酶的高效液相色谱（HPLC）分析表明分离出的酶是尿素酶。酶表现出在45的温度的最大活性^Ò C和pH为9。此外，粗酶表现出显着的方解石沉淀表示该菌株可用于微生物诱导的方解石沉淀（MICP），随后为生物粘结过程。