Rotavirus can be detected in stool samples by using serological and molecular methods. An easy and rapid dual gene RT-PCR assay for the simultaneous amplification of the capsid protein VP4 (structural) and rotavirus enter toxin NSP4 (non-structural protein) has been developed. We validated the assay by comparing the results of single target PCR with dual-gene PCR products.

中文翻译：

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开发双基因PCR检测试剂盒，可同时扩增牛轮状病毒A的结构和非结构基因

轮状病毒可以通过血清学和分子方法在粪便样本中检测到。已经开发了一种同时扩增衣壳蛋白VP4（结构性）和轮状病毒进入毒素NSP4（非结构性蛋白）的简便快速的双基因RT-PCR分析方法。我们通过比较单靶PCR和双基因PCR产物的结果验证了该检测方法。