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Pearl-necklace assembly of human serum albumin with the poly(acrylic acid) polyelectrolyte investigated using small angle X-ray scattering (SAXS)
Soft Matter ( IF 3.4 ) Pub Date : 2020-09-21 , DOI: 10.1039/d0sm01221a
Charaf E. Merzougui, Pierre Roblin, Pierre Aimar, Antoine Venault, Yung Chang, Christel Causserand, Patrice Bacchin

In this comprehensive study, the interaction of human serum albumin (HSA) with poly(acrylic acid) (PAA) was explored using small angle X-ray scattering (SAXS) combined with chromatography. The results revealed the formation of a complex between HSA macromolecules and PAA chains but solely under some specific conditions of the ionic strength and pH of the medium. In fact, this binding was found to take place only at pH close to 5 and at low ionic strength (0.15 M). Otherwise, for a higher pH and a salt concentration of 0.75 M the HSA–PAA complex tends to dissociate completely showing the reversibility of the complexation. The assessment of the influence of the HSA/PAA molar ratio on the radius of gyration of the complex suggests that 4 HSA molecules could bind to each 100 kDa PAA chain. In addition, the Porod volume evaluation for the same range of the HSA/PAA ratio confirms this assumption. Finally, an all-atom SAXS modelling study using the BUNCH program was conducted to find a compatible model that fits the HSA–PAA complex scattering data. This model allows us to portray the HSA/PAA complex as a pearl-necklace assembly with 4 HSA molecules on the 100 kDa PAA chain.

中文翻译:

使用小角度X射线散射(SAXS)研究人血清白蛋白与聚(丙烯酸)聚电解质的珍珠项链装配

在这项全面的研究中,使用小角度X射线散射(SAXS)与色谱法一起探讨了人血清白蛋白(HSA)与聚丙烯酸(PAA)的相互作用。结果表明,HSA大分子与PAA链之间形成了复合物,但仅在某些特定条件下,即离子强度和介质的pH值下。实际上,发现这种结合仅在接近5的pH和低离子强度(0.15 M)下发生。否则,对于较高的pH和0.75 M的盐浓度,HSA-PAA复合物趋于完全解离,表明该复合物具有可逆性。HSA / PAA摩尔比对复合物回转半径的影响评估表明,每个100 kDa PAA链上可以结合4个HSA分子。此外,在相同范围的HSA / PAA比值的Porod体积评估中证实了这一假设。最后,使用BUNCH程序进行了全原子SAXS建模研究,以找到适合HSA-PAA复杂散射数据的兼容模型。该模型使我们可以将HSA / PAA复合体描绘为一条珍珠项链装配体,在100 kDa PAA链上具有4个HSA分子。
更新日期:2020-10-11
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