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In vitro study of antibacterial and osteogenic activity of titanium metal releasing strontium and silver ions.
Journal of Biomaterials Applications ( IF 2.9 ) Pub Date : 2020-09-20 , DOI: 10.1177/0885328220959584
Yaichiro Okuzu 1 , Shunsuke Fujibayashi 1 , Seiji Yamaguchi 2 , Kazutaka Masamoto 1 , Bungo Otsuki 1 , Koji Goto 1 , Toshiyuki Kawai 1 , Takayoshi Shimizu 1 , Kazuaki Morizane 1 , Tomotoshi Kawata 1 , Yu Shimizu 1 , Makoto Hayashi 1 , Shuichi Matsuda 1
Affiliation  

Peri-prosthetic infection and loosening of implants are major problems in orthopaedic and dental surgery. To address these issues, surface treatment methods for titanium implants have been improved by modifying the alkali and heat treatment. We have previously fabricated calcium-treated Ti metal that releases Sr ions (CaSr-Ti), which resulted in a higher in vitro osteogenic response and early in vivo bone bonding.

Further, we developed a Ti metal that released both Sr and Ag ions (CaSrAg-Ti). In this study, we evaluated the antibacterial ability and osteogenic cellular response of CaSrAg-Ti and CaSr-Ti in vitro using rat bone marrow stromal cells (BMSCs) cultured on implant samples and extract mediums (EMs) made by immersing the implant samples in the medium. CaSrAg-Ti did not show cytotoxicity and was associated with a slightly higher osteogenic response when compared to CaSr-Ti, without inhibiting the effect of Sr. The osteogenic response was also observed in the cells cultured with the CaSrAg-Ti EM; however, the response was not as high as that of the cells on the CaSrAg-Ti implant sample. Significantly higher antibacterial activity was observed along with an antibacterial efficacy of more than 95% against methicillin-susceptible Staphylococcus aureus and Escherichia coli. The main advantages of our surface treatment are its simplicity and low cost. Therefore, our treatment is promising for clinical applications in orthopaedic or dental Ti-based implants with antibacterial and early bone-bonding abilities.



中文翻译:

钛金属释放锶和银离子的抗菌和成骨活性的体外研究。

假体周围感染和种植体松动是骨科和牙科手术中的主要问题。为了解决这些问题,钛种植体的表面处理方法已经通过改进碱和热处理来改进。我们之前制造了钙处理的钛金属,可释放 Sr 离子 (CaSr-Ti),从而导致更高的体外成骨反应和体内早期骨结合。

此外,我们开发了一种同时释放 Sr 和 Ag 离子的 Ti 金属 (CaSrAg-Ti)。在这项研究中,我们评估了 CaSrAg-Ti 和 CaSr-Ti的体外抗菌能力和成骨细胞反应使用在植入物样品上培养的大鼠骨髓基质细胞 (BMSC) 和通过将植入物样品浸入培养基制成的提取培养基 (EM)。CaSrAg-Ti 没有表现出细胞毒性,与 CaSr-Ti 相比,与略高的成骨反应相关,但不抑制 Sr 的作用。在用 CaSrAg-Ti EM 培养的细胞中也观察到成骨反应;然而,响应不如 CaSrAg-Ti 植入物样品上的细胞高。观察到显着更高的抗菌活性以及超过 95% 的对甲氧西林敏感的金黄色葡萄球菌大肠杆菌的抗菌功效. 我们表面处理的主要优点是简单和低成本。因此,我们的治疗有望用于具有抗菌和早期骨结合能力的骨科或牙科钛基植入物的临床应用。

更新日期:2020-09-21
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