Plants in the family Aristolochiaceae contain phenanthrene skeleton-containing chemical constituents that exhibit nephrotoxic, carcinogenic, mutagenic, anti-inflammatory, and cytotoxic effects. Two new phenanthrene-containing 1,2-oxazin-6-ones, designated as asaroidoxazine A (1) and asaroidoxazine B (2), and a known aristolactam, 5-methoxyaristololactam I (3), were isolated from the roots of Asarum asaroides. The structures of compounds 1 and 2 were determined using spectroscopic methods and X-ray crystallography. Treatment of SH-SY5Y human neuroblastoma cells with 1 μM of asaroidoxazine A (1) induced nuclear condensation as well as caspase-3/7 activation, indicating that this compound is a strong apoptosis inducer in neuronal cells. This is the first report of apoptosis induction by phenanthrene-containing oxazines.

中文翻译：

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细辛根中的细辛恶嗪诱导人神经母细胞瘤细胞凋亡。

马兜铃科植物含有含有菲骨架的化学成分，具有肾毒性、致癌性、致突变性、抗炎性和细胞毒性作用。从细辛的根中分离出两种新的含菲 1,2-oxazin-6-ones，命名为 asaroidoxazine A ( 1 ) 和 asaroidoxazine B ( 2 )，以及一种已知的马兜铃内酰胺，5-甲氧基马兜铃内酰胺 I ( 3 ) . 化合物1和2的结构使用光谱法和 X 射线晶体学确定。用 1 μM 的 asaroidoxazine A 处理 SH-SY5Y 人神经母细胞瘤细胞 ( 1) 诱导核凝聚以及 caspase-3/7 活化，表明该化合物是神经元细胞中的强凋亡诱导剂。这是含菲恶嗪诱导细胞凋亡的首次报道。