Gastric cancer (GC) is one of the most common malignancies of the digestive system with few genetic markers for its early detection and prevention. In this study, differentially expressed genes (DEGs) were analyzed using GEO2R from GSE54129 and GSE13911 of the Gene Expression Omnibus (GEO). Then, gene enrichment analysis, protein-protein interaction (PPI) network construction, and topological analysis were performed on the DEGs by the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, STRING, and Cytoscape. Finally, we performed survival analysis of key genes through the Kaplan-Meier plotter. A total of 1034 DEGs were identified in GC. GO and KEGG results showed that DEGs mainly enriched in plasma membrane, cell adhesion, and PI3K-Akt signaling pathway. Subsequently, the PPI network with 44 nodes and 333 edges was constructed, and 18 candidate genes in the network were focused on by centrality analysis and module analysis. Furthermore, data showed that high expressions of fibronectin 1(FN1), the tissue inhibitor of metalloproteinases 1 (TIMP1), secreted phosphoprotein 1 (SPP1), apolipoprotein E (APOE), and versican (VCAN) were related to poor overall survivals in GC patients. In summary, this study suggests that FN1, TIMP1, SPP1, APOE, and VCAN may act as the key genes in GC.

中文翻译：

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通过生物信息学分析鉴定胃癌关键基因

胃癌（GC）是消化系统最常见的恶性肿瘤之一，早期发现和预防的遗传标记很少。在这项研究中，差异表达基因（DEGs）使用了来自Gene Expression Omnibus（GEO）的GSE54129和GSE13911的GEO2R分析。然后，通过基因本体论（GO），京都基因与基因组百科全书（KEGG）途径，STRING和Cytoscape对DEG进行了基因富集分析，蛋白质-蛋白质相互作用（PPI）网络构建和拓扑分析。最后，我们通过Kaplan-Meier绘图仪对关键基因进行了生存分析。GC中总共鉴定出1034个DEG。GO和KEGG结果表明，DEG主要富集于质膜，细胞粘附和PI3K-Akt信号通路。后来，构建了具有44个节点，333条边的PPI网络，并通过集中度分析和模块分析关注了网络中的18个候选基因。此外，数据表明，纤连蛋白1（FN1），金属蛋白酶1（TIMP1）的组织抑制剂，分泌性磷蛋白1（SPP1），载脂蛋白E（APOE）和versican（VCAN）的高表达与GC总体生存期差有关耐心。总而言之，这项研究表明FN1，TIMP1，SPP1，APOE和VCAN可能是GC中的关键基因。和versican（VCAN）与GC患者的整体生存期差有关。总而言之，这项研究表明FN1，TIMP1，SPP1，APOE和VCAN可能是GC中的关键基因。和versican（VCAN）与GC患者的整体生存期差有关。总而言之，这项研究表明FN1，TIMP1，SPP1，APOE和VCAN可能是GC中的关键基因。