Abstract

This study was conducted to investigate the effect of taurine as a prophylactic treatment on antioxidant function and inflammatory responses of broilers challenged with lipopolysaccharide (LPS). A total of 256 one-day-old male Arbor Acres broiler chicks were randomly assigned to four treatments with eight replicates of eight birds (eight birds per cage). Four treatment groups were designated as follows: 1) in the CON group, broilers fed a basal diet; 2) in the LPS group, LPS-challenged broilers fed a basal diet; 3) in the LPS + T1 group, LPS-challenged broilers fed a basal diet supplemented with 5.0 g/kg taurine; and 4) in the LPS + T2 group, LPS-challenged broilers fed a basal diet supplemented with 7.5 g/kg taurine. The LPS-challenged broilers were intraperitoneally injected with 1 mg/kg body weight (BW) of LPS at 16, 18, and 20 d of age, whereas the CON group received an injection of sterile saline. The results showed that broilers injected with LPS exhibited decreased (P < 0.05) the average daily gain (ADG) and the 21-d BW (P < 0.05), while taurine supplementation alleviated the negative effects of LPS. Additionally, the LPS-induced increases (P < 0.05) in serum alanine transaminase and aspartate transaminase activities were reversed by taurine supplementation. The taurines could alleviate the hepatic oxidative stress, with the presence of lower content of malondialdehyde (P < 0.05), higher content of glutathione (P < 0.05), and an increased glutathione peroxidase (GSH-Px) activity (P < 0.05). The concentrations of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the liver were measured by ELISA kits, and the result showed that dietary taurine supplementation prevented these cytokines increases in the liver of LPS-induced broilers. Taurine reduced the genes expression of IL-1β, TNF-α, IL-6, cyclooxygenase-2, and inducible nitric oxide synthase, whereas it boosted the expression levels of antioxidant-related genes (nuclear factor erythroid 2-related factor 2, heme oxygenase-1, glutamate-cysteine ligase catalytic subunit, and GSH-Px) in the liver of LPS-induced broilers. In conclusion, dietary taurine supplementation in broilers mitigated LPS-induced defects in ADG, oxidative stress, and inflammatory responses.

中文翻译：

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膳食牛磺酸补充剂可减轻脂多糖诱导的肉鸡早期炎症反应和氧化应激。

摘要

本研究旨在研究牛磺酸预防性治疗对脂多糖 ( LPS )攻击的肉鸡的抗氧化功能和炎症反应的影响。总共 256 只 1 日龄雄性 Arbor Acres 肉鸡被随机分配到四个处理组，每组 8 只鸡重复 8 次（每笼 8 只鸡）。四个处理组指定如下： 1) 在CON 组中，肉鸡饲喂基础日粮；2)在LPS组中，LPS攻击的肉鸡饲喂基础日粮；3)在LPS+T1组中，LPS攻击的肉鸡饲喂添加了5.0g/kg牛磺酸的基础日粮；4）LPS+T2组，LPS 攻击的肉鸡饲喂补充 7.5 g/kg 牛磺酸的基础日粮。LPS 攻击的肉鸡在 16、18 和 20 日龄时腹腔注射 1 mg/kg 体重 ( BW ) 的 LPS，而 CON 组接受注射无菌生理盐水。结果表明，注射LPS的肉鸡表现出降低（P < 0.05）平均日增重（ADG）和21天体重（P < 0.05），而牛磺酸补充减轻了LPS的负面影响。此外，LPS 诱导的增加（P< 0.05) 血清丙氨酸转氨酶和天冬氨酸转氨酶活性被牛磺酸补充逆转。牛磺酸可以减轻肝脏氧化应激，丙二醛含量较低（P < 0.05），谷胱甘肽含量较高（P < 0.05），谷胱甘肽过氧化物酶（GSH-Px）活性增加（P < 0.05）。白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的浓度) 在肝脏中通过 ELISA 试剂盒进行测量，结果表明，膳食牛磺酸的补充阻止了 LPS 诱导的肉鸡肝脏中这些细胞因子的增加。牛磺酸降低了IL-1 β、TNF - α、IL-6、环氧合酶-2 和诱导型一氧化氮合酶的基因表达，同时提高了抗氧化相关基因（核因子红细胞 2 相关因子 2、LPS 诱导的肉鸡肝脏中的血红素加氧酶-1、谷氨酸-半胱氨酸连接酶催化亚基和GSH-Px )。总之，在肉鸡中添加牛磺酸可减轻 LPS 诱导的 ADG、氧化应激和炎症反应缺陷。