Abstract

This study investigated the effects of dietary osteopontin (OPN)-enriched algal protein on growth, immune status, and fecal fermentation profiles of weaned pigs challenged with a live infection of F18-fimbriated enterotoxigenic E. coli (ETEC). At 21 d of age, 54 pigs (5.95 ± 0.28 kg BW; blocked by BW) were allotted to 1 of 3 experimental groups combining dietary and health statuses. A control diet, containing 1% wild-type algal protein, was fed to both sham-inoculated (NC) and ETEC-inoculated (PC) pigs, while the test diet contained 1% OPN-enriched algal protein as fed only to ETEC-inoculated pigs (OA). All pigs received their assigned dietary treatment starting at study initiation to permit a 10-d acclimation period prior to inoculation. Growth performance, fecal dry matter, as well as hematological, histopathological, immune, and microbiota outcomes were analyzed by ANOVA, where treatment and time were considered as fixed effects and pig as a random effect; significance was accepted at P < 0.05. Overall, ETEC-inoculated pigs (PC and OA) exhibited decreased (P < 0.05) ADG and G:F, as well as increased (P < 0.05) peripheral blood helper T-cells and total leukocyte counts, compared with NC pigs during the postinoculation period. The OA treatment also elicited the highest (P < 0.05) concentrations of circulating tumor necrosis factor-α and volatile fatty acid concentrations in luminal contents at various postinoculation time-points, compared with other treatments. A principal coordinate analysis based on Unifrac weighted distances indicated that NC and OA groups had similar overall bacterial community structures, while PC pigs exhibited greater diversity, but infection status had no impact on α-diversity. Osteopontin-specific effects on microbial community structure included enrichment within Streptococcus and Blautia genera and decreased abundance of 12 other genera as compared with PC pigs. Overall, ETEC-infected pigs receiving 1% OPN-enriched algal protein exhibited changes immunity, inflammatory status, and colonic microbial community structure that may benefit weanling pigs experiencing F18 ETEC infection.

中文翻译：

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富含膳食骨桥蛋白的藻蛋白作为感染 F18 流苏产肠毒素大肠杆菌的断奶猪的营养支持。

摘要

本研究调查了富含骨桥蛋白 ( OPN )的膳食藻蛋白对受到 F18 流苏产肠毒素大肠杆菌( ETEC )活感染的断奶猪的生长、免疫状态和粪便发酵特征的影响。在 21 日龄时，将 54 头猪（5.95 ± 0.28 kg BW；被 BW 阻止）分配到 3 个结合饮食和健康状况的实验组中的 1 个。将含有 1% 野生型藻蛋白的对照日粮喂给假接种 ( NC ) 和 ETEC 接种 ( PC ) 的猪，而测试日粮含有 1% 富含 OPN 的藻蛋白，仅喂给 ETEC-接种猪（OA））。所有猪在研究开始时都接受了指定的饮食治疗，以便在接种前有 10 天的适应期。通过方差分析分析生长性能、粪便干物质以及血液学、组织病理学、免疫和微生物群结果，其中处理和时间被视为固定效应，猪被视为随机效应；在P < 0.05时接受显着性。总体而言，与 NC 猪相比，接种 ETEC 的猪（PC 和 OA）的ADG 和 G:F降低（P < 0.05），外周血辅助 T 细胞和总白细胞计数增加（P < 0.05）。接种后时期。OA 治疗也引起最高（P< 0.05) 与其他治疗相比，在不同的接种后时间点，循环肿瘤坏死因子-α 的浓度和管腔内容物中的挥发性脂肪酸浓度。基于 Unifrac 加权距离的主坐标分析表明，NC 和 OA 组具有相似的整体细菌群落结构，而 PC 猪表现出更大的多样性，但感染状态对 α-多样性没有影响。骨桥蛋白对微生物群落结构的特异性影响包括在链球菌和Blautia 中的富集与 PC 猪相比，其他 12 个属的丰度降低。总体而言，接受 1% OPN 富集藻蛋白的 ETEC 感染猪表现出免疫、炎症状态和结肠微生物群落结构的变化，这可能有益于经历 F18 ETEC 感染的断奶猪。