Abstract

This study was conducted to explore the effect of the zinc oxide nanoparticles (ZnONPs) supplement on the regulatory appetite and heat stress (HS) genes in broiler chickens raised under high or normal ambient temperatures. In this study, 240 one-day-old male broiler chicks (Cobb 500) were randomly assigned to 48 battery cages. From day 1, these 48 cages were randomly subjected to four different treatment strategies: Control (wherein, their basal diet included 60 mg/kg of ZnO), ZNONPs 40 (wherein basal diet included 40 mg/kg of ZnONPs), ZnONPs 60 (basal diet included 60 mg/kg of ZnONPs), and ZnONPs 100 (basal diet included 100 mg/kg of ZnONPs). Thereafter, from day 22 to 42, the chickens from each dietary treatment group were subjected to different temperature stresses either normal (23 ± 1 °C constant) or HS (34 ± 1 °C for 6 h/d), which divided them into eight different treatment groups. Our findings revealed that dietary ZnONPs altered the gene expression of cholecystokinin (ileum), heat stress proteins (HSP) 70 (jejunum and ileum), and HSP 90 (duodenum, jejunum, and ileum). The gene expression of ghrelin was affected by the interaction between the ZnONPs concentration and temperature in the duodenum and stomach. More studies are required to elucidate its complex physiological and biochemical functions of the regulation of gene expression within the intestine in heat-stressed broiler chickens.

中文翻译：

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肉鸡中的锌离子和基因表达氧化锌纳米颗粒对热应激下肉鸡的食欲调节和热应激蛋白基因的影响。

摘要

本研究旨在探讨氧化锌纳米颗粒（ZnONPs）补充剂对调节食欲和热应激（HS）的影响。) 在高温或正常环境温度下饲养的肉鸡中的基因。在这项研究中，240 只 1 日龄雄性肉鸡 (Cobb 500) 被随机分配到 48 个电池笼中。从第 1 天起，这 48 个笼子随机接受四种不同的处理策略：对照（其中，它们的基础饮食包括 60 mg/kg ZnO）、ZNONPs 40（其中基础饮食包括 40 mg/kg ZnONPs）、ZnONPs 60（基础饮食包括 60 mg/kg ZnONPs）和 ZnONPs 100（基础饮食包括 100 mg/kg ZnONPs）。此后，从第 22 天到第 42 天，来自每个日粮处理组的鸡经受不同的温度应激，正常（23±1°C 恒定）或 HS（34±1°C，持续 6 小时/天），将它们分为八个不同的治疗组。我们的研究结果表明，膳食 ZnONPs 改变了胆囊收缩素（回肠）的基因表达，HSP ) 70（空肠和回肠）和 HSP 90（十二指肠、空肠和回肠）。ghrelin的基因表达受十二指肠和胃中ZnONPs浓度与温度相互作用的影响。需要更多的研究来阐明其在热应激肉鸡肠道内调控基因表达的复杂生理生化功能。