The supplementation of ruminant diets with exogenous cellulolytic enzymes can improve their digestibility and feeding value. The objective of this study was to determine the effect of supplementing roughage (rice straw) and concentrate with inoculants containing four fungal strains (Pleurotus ostreatus, Phanerochaete chrysosporium, Trichoderma reesei and Trichoderma viride) and four bacterial strains (Paenibacillus polymyxa, Bacillus megaterium, Bacillus circulans and Bacillus subtilis), given separately or as a mixture, as a source of exogenous cellulolytic enzymes, on basic rumen parameters in vitro, including digestibility and methane production. A batch culture trial was used to select the best supplements, and a long-term rumen simulation technique (RUSITEC) was used to evaluate the effects of P. chrysosporium, B. subtilis, and a 1 : 1 mixture of these two on dietary component digestibility and fermentation parameters. In the batch culture evaluation, there were significant increases in the organic matter (OM) digestibility, the total gas production expressed as ml/g of dry matter, the OM, the neutral detergent fibre (NDF) and the acid detergent fibre (ADF) of the supplemented rations, as compared to the control, excluding the rations supplemented with T. viride and B. circulans. In the RUSITEC, the ration supplemented with mixed inoculants showed significantly higher digestibility of crude protein, ether extract, NDF and ADF than did the ration supplemented with the P. chrysosporium and B. subtilis inoculants. It can be concluded that the simultaneous use of fungal and bacterial exogenous cellulases on rice straw roughage improves its digestibility, without negative effects on other rumen parameters.

反刍动物日粮中添加外源纤维素分解酶可以提高其消化率和摄食价值。这项研究的目的是确定补充粗饲料（稻草）并用包含四种真菌菌株（平菇，平生黄单孢菌，里氏木霉和里氏木霉）和四种细菌菌株（多粘芽孢杆菌，巨大芽孢杆菌，芽孢杆菌，芽孢杆菌）的接种物浓缩的效果。圆环菌和枯草芽孢杆菌（Bacillus subtilis），作为外源纤维素分解酶的来源，分别或以混合物形式提供，基于体外基本瘤胃参数，包括消化率和甲烷产量。分批培养试验被用于选择最佳的补充剂，和使用了长期瘤胃模拟技术（RUSITEC）评价的影响黄孢原毛平革，枯草芽孢杆菌，和一个1：这两个对膳食组分1的混合物消化率和发酵参数。在分批培养评估中，有机物（OM）的消化率显着提高，总气体产量以干物质的毫升/克，OM，中性洗涤剂纤维（NDF）和酸性洗涤剂纤维（ADF）表示。与对照组相比的补充口粮中，不包括补充了维耶螺旋体和马来酸杆菌的口粮。在RUSITEC中，添加了混合接种剂的日粮显示出的粗蛋白，乙醚提取物，NDF和ADF的消化率明显高于添加金黄色葡萄球菌和枯草芽孢杆菌接种物的日粮。可以得出结论，在稻草粗料上同时使用真菌和细菌外源纤维素酶可以提高其消化率，而对其他瘤胃参数没有负面影响。