Although many studies have assessed the various effects of garlic on fish, the impact of garlic on steroid hormones has not been studied. In the present study, the effect of aqueous garlic extract on sex steroid hormones, fry production, digestive enzyme activities, growth performance, and survival of Poecilia reticulata were evaluated. A total of 360 P. reticulata fries (0.013 ± 0.001 g) were randomly stocked in 12 experimental glass tanks (50 L) at a density of 30 fish per tank. The fries were fed diets supplemented with aqueous garlic extract at 0 (Control), 1.00 (G1), 1.50 (G2), and 2.00 (G3) ml/kg diet three times a day at a ratio of 2.5% of their body weight for a period of 120 days. Results demonstrated that the inclusion of garlic extract in the diet did not have any significant effect on growth performance and fry production. The survival of G2 treatment was significantly higher than the control, G1 and G3 (p < .05). The concentrations of sex steroid hormones (estradiol, testosterone, 17α‐hydroxyprogesterone and progesterone) and the activity of digestive enzymes (protease, lipase, and amylase) were significantly higher in G2, than the control and other treatments (p < .05). Based on polynomial regression models, the recommended dietary concentration of garlic extract for P. reticulata should be 1.64 ± 0.03 and 1.63 ± 1.01 ml/kg to obtain higher activities of digestive enzymes and sex steroids, respectively.

中文翻译：

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补充了大蒜（大蒜）提取物的孔雀鱼（Poecilia reticulata）的性参数，消化酶活性和生长性能

尽管许多研究评估了大蒜对鱼类的各种影响，但尚未研究大蒜对类固醇激素的影响。在本研究中，评估了大蒜水提取物对性类固醇激素，鱼苗产量，消化酶活性，生长性能和网状Poecilia存活的影响。总共360个网状假单胞菌将鱼苗（0.013±0.001 g）随机放入12个实验玻璃罐（50 L）中，每罐密度为30条鱼。每天以0（对照），1.00（G1），1.50（G2）和2.00（G3）ml / kg的饮食饲喂补充了大蒜含水提取物的饲料，其比例为体重的2.5％，为期120天。结果表明，日粮中包含大蒜提取物对生长性能和鱼苗生产没有任何显着影响。G2治疗的存活率显着高于对照组，G1和G3（p <.05）。G2中性类固醇激素（雌二醇，睾丸激素，17α-羟基孕酮和孕酮）的浓度和消化酶的活性（蛋白酶，脂肪酶和淀粉酶）显着高于对照和其他治疗方法（p  <.05）。基于多项式回归模型，网纹假单胞菌的大蒜提取物的建议饮食浓度应为1.64±0.03和1.63±1.01 ml / kg，以获得更高的消化酶和性类固醇活性。