Global warming increases seawater temperature, causing high temperature stress to marine organisms, including algae. This study aimed to explore the global proteomic response of Sargassum fusiforme under high temperature stress. Sargassum fusiforme seedlings were cultured in natural seawater for 24 hr and subjected to different temperatures (22°C, control group; 27°C and 32°C, high temperature stress group) for 1, 3, 5 and 7 days. Changes in their membrane lipid peroxidation after high temperature stress were investigated. Proteomic changes in the air bladders of S. fusiforme were analysed using isobaric tags for relative and absolute quantification, along with liquid chromatography–tandem mass spectrometry. Data were analysed using bioinformatics methods. Results showed that high temperature stress destroyed the cell membrane of the air bladders. Further, 28 and 53 differentially expressed proteins (DEPs) were found in the 27°C and 32°C treatment groups respectively. These DEPs were mainly involved in glycolysis, single‐organism catabolism, purine nucleoside diphosphate metabolism and carbohydrate catabolism. In addition, DEPs were significantly enriched in 10 pathways, such as glycolytic process, biosynthesis of antibiotics, ribosome, biosynthesis of secondary metabolites and biosynthesis of amino acids. Proteomics analyses indicated that proteins associated with synthesis, folding, degradation, photosynthesis and energy and carbohydrate metabolism are differentially expressed under high temperature stress and normal conditions.

中文翻译：

---

基于iTRAQ的羊栖菜响应高温胁迫的定量蛋白质组学分析。

全球变暖升高了海水温度，对包括藻类在内的海洋生物造成了高温胁迫。本研究旨在探讨高温胁迫下羊栖菜（Sargassum fusiforme）的整体蛋白质组反应。羊栖菜幼苗在天然海水中培养24小时，然后经受不同的温度（22℃，对照组； 27℃和32℃，高温胁迫组）1、3、5和7天。研究了高温胁迫后它们膜脂过氧化的变化。梭状孢子囊中蛋白质组学的变化使用等压标记对相对和绝对定量进行分析，并进行液相色谱-串联质谱分析。使用生物信息学方法分析数据。结果表明，高温胁迫破坏了气囊的细胞膜。此外，在27°C和32°C处理组中分别发现28和53个差异表达蛋白（DEP）。这些DEP主要参与糖酵解，单生物分解代谢，嘌呤核苷二磷酸代谢和碳水化合物分解代谢。此外，DEPs在糖酵解过程，抗生素的生物合成，核糖体，次生代谢产物的生物合成和氨基酸的生物合成等10种途径中均显着丰富。蛋白质组学分析表明，与合成，折叠，降解，