Employing a sequentially activated probe design method, an activatable, switchable and dual‐mode probe was designed. This nanoprobe, HSDPP, could be effectively activated by H₂S to form H‐type aggregates with green emission; subsequently, the aggregates could bind to mtDNA to form monomers and the emIssion color switched from green to deep‐red. We exploited HSDPP to image exogenous and endogenous H₂S in living cells. Of note, for the first time, this novel nanoprobe with an optimal partition coefficient value (LogP=1.269) was successfully applied for tracking the endogenous H₂S upregulation stimulated by cystathionase activator S‐adenosyl‐L‐methionine (SAM) in mice brains. Finally, our work provides an invaluable chemical tool for probing endogenous H₂S upregulation in vitro/vivo and, importantly, affords a prospective design strategy for developing switchable chemosensors to unveil the relationship between biomolecules and DNA in mitochondria in many promising areas.

中文翻译：

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一种用于检测 H2S 的新型可激活和可切换纳米聚集探针及其在小鼠大脑中的应用。

采用顺序激活的探头设计方法，设计了一种可激活、可切换的双模式探头。这种纳米探针HSDPP可以被H ₂ S有效激活，形成具有绿色发射光的H型聚集体；随后，聚集体可以与 mtDNA 结合形成单体，并且发射颜色从绿色变为深红色。我们利用HSDPP对活细胞中的外源性和内源性 H ₂ S进行成像。值得注意的是，这种具有最佳分配系数值 (LogP=1.269) 的新型纳米探针首次成功应用于跟踪胱硫醚酶激活剂S-腺苷-L刺激的内源性 H ₂ S 上调‐小鼠大脑中的甲硫氨酸 (SAM)。最后，我们的工作提供了一种宝贵的化学工具，用于在体外/体内探测内源性 H ₂ S 上调，重要的是，为开发可切换的化学传感器提供了一种前瞻性的设计策略，以揭示许多有前途的领域中线粒体中生物分子与 DNA 之间的关系。